Neuropilin-1在喉癌组织及喉癌细胞系中的表达

目的：探讨Neuropilin-1（NRP-1）在喉癌组织及喉癌细胞系中的表达及意义。方法：采用免疫组织化学法检测NRP-1在喉癌及癌旁组织中的表达；采用RT-PCR检测NRP-1mRNA在喉癌、癌旁组织及喉癌细胞系中的表达。结果：免疫组织化学检测发现28例喉癌组织标本中NRP-1均为高表达，而相应的癌旁组织则无表达；RT-PCR检测NRP-1mRNA的表达结果显示28例喉癌组织中24例呈阳性表达，Hep-2细胞系呈阳性表达，相应的癌旁组织为阴性表达。结论：NRP-1表达于大多数喉癌组织及喉癌细胞系Hep-2中，而在癌旁组织不表达，提示NRP-1的表达可能与喉癌的发生、发展密切相关，且有较高的肿瘤特异性。

Expression of Neuropilin-1 in human laryngeal carcinoma and cell lines

Objective:To study the expression of Neuropilin-1 in human carcinoma and papilloma of larynx specimens and carcinoma cell lines Hep-2.Method:The expression of Neuropilin-1 in human laryngeal carcinoma,papilloma specimens were investigated by immunohistochemical staining; the expression of Neuropilin-1 mRNA in human laryngeal squamous cell carcinoma, papilloma specimens and cell lines were examined by using RT-PCR.Result:Immunohistochemical analysis revealed that NRP-1 was expressed in all human laryngeal squamous cell carcinoma specimens but was absent in nonmalignant laryngeal tissue. The mRNA of NRP 1and Hep-2 cell lines are positive in 24 of 28 human laryngeal carcinoma specimens but not negative in nonmalignant laryngeal specimens.Conclusion:Neuropilin-1 was expressed in most of human laryngeal carcinoma specimens and cell lines but not expressed in nonmalignant tissue. It suggests that the expression of Neuropilin-1 may be related to the risk and development of laryngeal carcinoma and has high distinguishability.