组胺在豚鼠颈上神经节投射到心脏交感神经纤维中的表达

目的 探讨组胺在豚鼠颈上神经节投射到心脏交感神经纤维中的表达,及其与去甲肾上腺素的共存,为组胺可能是心脏交感神经系统新发现的递质提供形态学证据. 方法生物索化葡聚糖胺(BDA)顺行追踪结合组胺和去甲肾上腺素免疫荧光三标技术. 结果将BDA注入颈上神经节后,左、右心房和心室均有其投射的交感神经纤维,同时在这些示踪纤维中亦有组胺和组胺/去甲肾上腺素共存的免疫阳性表达. 结论豚鼠颈上神经节投射到心脏交感神经纤维中有组胺的表达并与去甲肾上腺紊共存.

THE EXPRESSION OF HISTAMINE IN THE CARDIAC SYMPATHETIC FIBERS FROM THE GUINEA PIG SUPERIOR CERVICAL GANGLION

Objective To investigate the expression of histamine in the cardiac sympathetic fibers from the guinea pig superior cervical ganglion and its coexistence with norepinephrine so as to provide morphological evidence for histamine as a cardiac sympathetic neurotransmitter. Methods Biotinylated dextranamine (BDA) anterograde tracing and immunofluorescence histochemical staining for histamine/norepinephrine were applied. Results After injection of BDA into the superior cervical ganglion, BDA labeled sympathetic fibers in the left and right atria and ventricle were observed. Meanwhile, the tracing fibers proved histamine-like immunoreactive or both histamine and norepinephrine-like immunoreactive. Conclusion Histamine is expressed in the cardiac sympathetic fibers from the guinea pig superior cervical ganglion and coexisted with norepinephrine.