新生儿社区获得性肺炎与血清25羟维生素D、淋巴细胞亚群关系分析

目的 分析新生儿社区获得性肺炎患儿血清25羟维生素D水平及淋巴细胞亚群变化,为临床治疗提供科学依据。方法 随机选取2018年11月-2019年1月在永康市第一人民医院诊断为社区获得性肺炎的68例足月新生儿为观察组,同期在本院产科出生的68例健康足月新生儿为对照组。采用BIO-RAD 680酶标仪ELISA法检测血清25羟维生素D水平及采用BD公司Facs Canto2流式细胞仪检测淋巴细胞亚群。结果 观察组与对照组比较,观察组血清25羟维生素D水平明显低于对照组,差异有统计学意义(t=6.815,P＜0.01)。观察组CD3+ 和CD4+细胞阳性率以及CD4+/CD8+比值,均显著低于照组(t=2.912、2.947、3.115,P＜0.01);观察组CD19+细胞阳性率显著高于对照组(t=5.565,P＜0.01)。观察组中25羟维生素D 缺乏组与正常组比较,缺乏组CD3+和CD4+细胞阳性率以及CD4+/CD8+,均显著低于正常组(t=3.084、4.234、3.021,P＜0.01);而缺乏组CD19+细胞阳性率显著高于正常组(t=3.515,P＜0.01)。结论 社区获得性肺炎新生儿的25羟维生素D缺乏发生率较高;社区获得性肺炎新生儿细胞免疫水平偏低,可能与25维生素D水平低有关。

Analysis of the relationship between neonatal community acquired pneumonia and serum 25-hydroxyvitamin D and lymphocyte subsets

Objective To analysis the serum 25-hydroxyvitamin D level and lymphocyte subsets in neonates with community acquired pneumonia,and to provide evidence for clinical treament. Methods A total of 68 full-term neonates diagnosed with community acquired pneumonia in the First People′s Hospital of Yongkang City from November 2018 to January 2019 were randomly selected as the observation group,and 68 healthy full-term neonates born in the same period of our hospital as the control group.Serum 25-hydroxyvitamin D level was detected by BIO-RAD 680 ELISA and lymphocyte subsets were detected by Facs Canto2 flow cytometry of BD company. Results Compared with the control group,the serum 25-hydroxyvitamin D level in the observation group was significantly lower than that in the control group (t=6.815,P＜0.01).The positive rate of CD3+ and CD4+ cells and the ratio of CD4+/CD8+ in the observation group were significantly lower than those in the control group (t=2.912,2.947,3.115,P＜0.01); the positive rate of CD19+ cells in the observation group was significantly higher than that in the control group (t=5.565, P＜0.01).The positive rates of CD3+ and CD4 + cells,CD4+/CD8+ in the 25-hydroxyvitamin D deficiency group were significantly lower than those in the normal group (t=3.084,4.234,3.021,P＜0.01); but the positive rates of CD19+ cells in the deficient group was significantly higher than that in the normal group (t=3.515, P＜0.01). Conclusionss The incidence of 25-hydroxyvitamin D deficiency in neonates with community-acquired pneumonia is higher.The level of cellular immunity in neonates with community-acquired pneumonia is lower,which may be related to the low level of 25-vitamin D.