甘露糖对6个人非小细胞肺癌细胞系放射敏感性影响

目的探讨甘露糖对6个人非小细胞肺癌细胞系放射敏感性的影响及其可能机制。方法采用Western blot检测磷酸甘露糖异构酶在6个肺癌细胞系中的表达。利用MTT法观察甘露糖对肺癌细胞系的增殖抑制作用;分别给予对照组、甘露糖组0、2、4、6、8、10 Gy照射,采用平板克隆形成实验检测甘露糖对6个肺癌细胞放射敏感性的影响;流式细胞术检测对照组、甘露糖组、照射组及联合组细胞的凋亡情况。结果6个肺癌细胞系中磷酸甘露糖异构酶表达量各不相同,其中A549细胞表达量最高,H460细胞表达量最低。11.1 mmol/L甘露糖对A549、H460细胞系抑制作用相同,随着甘露糖浓度增加,对H460细胞系抑制作用更显著。采用11.1 mmol/L的甘露糖可明显增加H460细胞系放射敏感性及细胞凋亡率,而对A549细胞系放射敏感性和凋亡率影响不大。结论在磷酸甘露糖异构酶高表达的6个肺癌细胞系中,甘露糖可增强部分肿瘤细胞的放射敏感性。

Effect of mannose on the radiosensitivity of six non-small cell lung cancer cell lines

Objective To investigate the effect of mannose on the radiosensitivity of six human non-small cell lung cancer cell lines and its possible mechanism. Methods The expression of mannose phosphate isomerase in six lung cancer cell lines were detected by Western blot. The inhibitory effect of mannose on the proliferation of lung cancer cell lines were observed by MTT assay. When irradiated with 0, 2, 4, 6, 8 and 10Gy, the effect of mannose on the radiosensitivity of six lung cancer cell lines was detected by plate clone formation assay, respectively;and the apoptosis rates of normal control, mannose, irradiation and combined groups were detected by flow cytometry. Results The expression levels of mannose phosphate isomerase were different among six lung cancer cell lines. Among them, A549 cells had the highest expression level and H460 cells showed the lowest expression level. When aDministrated with 11.1 mmol/L mannose, the same inhibitory effect was observed on both A549 and H460 cell lines. Moreover, the inhibitory effect on H460 cell line was significantly increased with the increase of mannose concentration. In addition, aDministration of 11.1 mmol/L mannose could significantly increase the radiosensitivity and apoptosis rate of H460 cell line. However, it exerted limited effect upon the radiosensitivity and apoptosis rate of A549 cell line. Conclusion In six lung cancer cell lines with high expression of mannose phosphate isomerase, the aDministration of mannose can enhance the radiosensitivity of partial tumors cells.