正常食蟹猴不同适应时间视网膜电图的 变化

目的：研究明/暗适应时间对正常成年食蟹猴闪光视网膜电图（ERG）结果的影响。方法：实验研究。 选取3只成年食蟹猴，分别于暗适应20、40、60 min后用国际临床视觉电生理会标准参数刺激，常 规程序记录暗适应ERG，在暗适应40 min后，分别明适应1、5、10 min记录明适应ERG。采用单因 素方差分析比较各时间段振幅与潜伏期变化。结果：不同暗适应时间，a、b波和震荡电位OPs的潜 伏期差异均无统计学意义（F=0.052，P=0.949），暗适应时间小于40 min时，a、b波和OPs的振幅随暗 适应时间增加而显著增大（F=50.800，P<0.001）；暗适应40 min时，振幅达到最大，且不再随暗适应 时间增加而增大（F=0.016，P=0.899）。明适应a、b波的潜伏期同样不受明适应时间的影响（F=0.980， P=0.381），a、b波的振幅在明适应5 min后达到最大（F=4.789，P=0.036）且随时间延长趋于平稳 （F=0.135，P=0.717）。结论：在一定时间内，明/暗适应时间对食蟹猴闪光ERG结果影响较大，故对 食蟹猴暗适应ERG记录的时间不宜少于40 min，而明适应ERG在5 min后即可记录。

Changes in the Electroretinogram in Normal Cynomolgus Monkeys at Different Adaptation Times

Objective: To study the effects of light/dark adaptation times on flash electroretinograms in normal adult cynomolgus monkeys. Methods: In this experimental study, three adult cynomolgus monkeys were selected and stimulated with standard electroretinogram (ERG) parameters after dark adaptation for 20 min, 40 min and 60 min. The scotopic-ERG was recorded using aroutine procedure. After dark adaptation for 40 min, the photopic-ERG was recorded for 1 min, 5 min and 10 min. One-way ANOVA was used to compare the amplitude and latency. Results: There was no significant difference in the latencies of a and b waves and OPs in the scotopic-ERG (F=0.052, P=0.949). When the dark-adaption time was less than 40 min, the amplitudes of the a and b waves and OPs increased significantly as the adaptation time increased (F=50.800, P<0.001). When the dark-adaptation time was more than 40 min, the amplitude reached the maximum and no longer increased with time (F=0.016, P=0.899). For the photopic-ERG, the implicit times of the a and bwaves were also unaffected by the length of the light-adaptation time (F=0.980, P=0.381). The amplitudes of the a and b waves reached their maximum after 5 minutes of light adaptation (F=4.789, P=0.036) and tended to be stable over time (F=0.135, P=0.717). Conclusions: In a certain period of time, the results of flash ERG of cynomolgus monkeys are greatly affected by bright/dark time. So, the dark-adaptation time for scotopic-ERG recording in cynomolgus monkeys should be at least 40 minutes, and the photopic-ERG are best recorded after 5 minutes of light adaption.