高效液相色谱法测定牛白藤中齐墩果酸和熊果酸含量

目的 建立以高效液相色谱法(HPLC)同时测定牛白藤药材中齐墩果酸和熊果酸含量的方法.方法 色谱柱为Diamonsil C18 (4.6 mm×250 mm,5μm),流动相为甲醇:0.1％磷酸(80:20),流速为1.0 mL/min,柱温为30℃,检测波长为210 nm.结果 齐墩果酸和熊果酸分别在0.0910～0.9100 μg(r=0.999 8)和0.084 6～0.821 8 μg(r=0.999 7)呈良好线性关系,二者平均加样回收率分别为99.91％、98.38％,相对标准偏差(RSD)分别为2.5％、2.2％.结论 高效液相色谱法简便、准确、可靠,可用于牛白藤的质量控制.

Determination of oleanolic acid and ursolic acid in Hedyotis hedyotidea by HPLC

Objective To establish a HPLC method for simultaneous determination of oleanolic acid and ursolic acid in Hedyotis hedyotidea.Methods The analytical column was Diamonsil C18 column（4.6 mm×250 mm,5μm）.The mobile phase consisted of methanol-0.1% phosphoric acid（80 ∶ 20）.The flow rate was 1.0 mL/min,the column temperature was set at 30 ℃ and the detection wavelength was 210 nm.Results The calibration curves of oleanolic acid and ursolic acid showed good linearity in the range of 0.091 0-0.910 0 μg（r=0.999 8） and 0.084 6-0.821 8 μg（r=0.999 7）,respectively.Their average recoveries were 99.91% and 98.38%,RSD 2.5%（n=6）and 2.2%（n=6）,respectively.Conclusion The established method is simple,accurate and reliable,which can be used for the quality control of Hedyotis hedyotidea.