免疫组织化学双标记结合激光显微切割技术在霍奇金淋巴瘤诊断中的应用

 目的 探讨IHC双标记技术结合LCM技术在研究HL肿瘤细胞与背景细胞中的应用。 方法 选取10例HL标本,10%中性福尔马林固定,石蜡包埋组织,采用不同的抗原修复方法,通过IHC 双标记与LCM技术获得单一的肿瘤细胞和背景细胞。 结果 在10例HL标本中通过IHC双标记与LCM技术获得单一的肿瘤细胞和背景细胞,二者表现出不同的 颜色以及表达不同的CD分子。 结论 通过IHC双标记与LCM技术获得单一的肿瘤细胞和背景细胞,IHC双标记在HL研究中起着区分不 同细胞的作用。

Application of Combination of Double-labelling with Immunohistochemistry and Laser Capture Microdissection in Hodgkin's Lymphoma

Objective To evaluate the Application of detecting Hodgkin's Lymphoma(HL) tumor cell and background cell with the combination of double-labeling with immunohistochemistry (IHC) and Later Capture microdissection (LCM).Methods This study includes 10 patients with HL diagnosed,tissue biopsy sample were fixed 10% formalin, paraffin-embedded, adopted different methods of antigen retrieval.The same sample were labeled with double labeling with IHC(CD20/CD45RO, CD30), then single tumor cell and background cell can get through LCM.Results In 10 cases of HL, tumor cells and background cells were detected with the combination of double labeling and LCM, they might show different color.The expression of tumor cell was CD30-postive with brown-yellow on cell membrane, and background cells were CD20-positive with red on cell membrane.Conclusion We can tell single tumor cells and background cell with double labeling and LCM, thus double labeling might play distinguish between different cells in HL.