不同剂量肾上腺素对大鼠心肺复苏的影响

目的]探讨不同剂量肾上腺素对大鼠心肺复苏的影响.方法]选用清洁级雄性SpragueDawley（SD）大鼠32只分为两组,采用呼气末夹闭气管窒息致大鼠心脏骤停,夹闭5 min后开始心肺复苏,给予不同剂量肾上腺素（0.02 mg/kg,小剂量组;0.2 mg/kg,大剂量组）,记录心率、血压的变化,自主循环恢复（ROSC）的时间,1 h和6 h存活率.结果]造模后约1～2 min所有大鼠均出现心脏骤停,小剂量组与大剂量组复苏成功率分别为69%和74%,ROSC时间分别为（155±78）s、（85±25）s,1 h存活率分别为89%和86%,6 h存活率分别为44%和57%.两组复苏成功率,6 h存活率相比较差异无显著性（P〉0.05）;而1 h存活率,ROSC时间相比较差异有显著性（P〈0.05）.结论]0.2 mg/kg肾上腺素用于大鼠心肺复苏较0.02 mg/kg可明显减少心脏按压时间,缩短ROSC时间,但对提高复苏成功率和6 h存活率无明显差异,反而会降低1 h存活率.

Effect of Different Doses of Epinephrine on Cardiopulmonary Resuscitation in Rats

Objective] To explore the effect of different doses of epinephrine on cardiopulmonary resuscitation（CPR） in rats. Methods] Thirty-two male Sprague-Dawley （SD） rats were randomly divided into two groups. The animal models of cardiac arrest（CA） were made by clamping the trachea at end expiration. CPR was initiated after 5 minutes. Meanwhile, the rats were injected respectively different doses of epinephrine（low dose of 0.02mg/kg and high dose of 0.2mg/kg）. Heart rate, blood pressure, the time of arrest and restoration of spontaneous circulation（ROSC） and 1-hour and 6-hour survival rate were recorded. Results] CA in all rats appeared 1- 2 minutes after the establishment of the rat model. The successful rate of resuscitation in low-dose group and high-dose group was 69% and 74%, respectively. The time of ROSC in low-dose group and high-dose group was （155q-78）s and （85-}-25）s, respectively. The 1-hour survival rate in low-dose group and high-dose group was 89% and 86%, respectively. The 6-hour survival rate in low-dose group and highdose group was 44% and 57 %, respectively. There was no significant difference in the successful rate of resuscitation and 6-hour survival rate between two groups（ P〉0.05）, while there was significant difference in 1-hour survival rate and the time of ROSC between two groups（ P d0.05）. Conclusion] Epinephrine 0.2mg/ kg can obviously reduce the cardiac compression time and shorten the time of ROSC. But there is no obvious difference in improving the successful rate of resuscitation and 6-hour survival rate between high-dose and low- dose epinephrine. On the contrary, epinephrine 0.2mg/kg may decrease 1-hour survival rate.