奥曲肽对肝癌细胞SMMC-7721增殖和凋亡的影响

目的：探讨生长抑素类似物奥曲肽（octreotide）对人肝癌细胞SMMC－7721增殖和凋亡的影响。方法：用MTT比色法分析octreotide对肝癌细胞生长的影响，用流式细胞仪检测细胞凋亡率，细胞周期分布及凋亡相关基因p53,bcl-2的表达。结果：octreotide对人肝癌细胞SMMC－7721的增殖存在抑制作用，与对照相比有明显差别（P＜0．01），这种作用表现出量效及时效关系。流式细胞仪检测发现：octreotide处理组肝癌细胞凋亡率升高，p53基因表达增强，bcl-2表达下降，细胞从G1期进入S期受阻。结论：生长抑素类似物octreotide可抑制肝癌细胞SMMC－7721的增殖，并通过影响细胞的周期分布及凋亡相关基因p53、bcl-2的表达，诱导肝癌细胞的凋亡。

The Effect of Octreotide on the Proliferation and Apoptosis of Human Hepatocellular Carcinoma Cell SMMC-7721

Objective:To investigate the effect of octreotide on the proliferation and apoptosis of hepatocellular carcinoma cell SMMC-7721.Method:The effects of octreotide on human hepatocellular carcinoma cell SMMC-7721 were studied by using MTT.Flow cytometry(FCM)analysis was carried out to quantify cellular apoptosis,proliferation index(PI),the expression of apoptosis related gene p53?bcl-2,and to determine the changes of cell cycle.Results:octreotide had a dose-dependent and time-dependent antiproliferation effect on SMMC-7721 cells,compared with control group,and had significant difference(P<0.01).FCM revealed that bcl-2 were down-regulated,while p53 up-regulated in octreotide group.The elevation of apoptosis peak were observed octreotide group.Conclusion:Octreotide exert inhibiting effect on human hepatocellular carcinoma cell SMMC-7721,and induce apoptosis in SMMC-7721 cells.Octreotide may have a potential value in clinically treating hepatocellular carcinoma.