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| 采用第一极体进行植入前诊断的实验研究 | |
| 引用本文： | 陈雯,朱桂金,Mettler L.采用第一极体进行植入前诊断的实验研究[J].现代妇产科进展,2004,13(5):333-335. |
| 作者姓名： | 陈雯 朱桂金 Mettler L |
| 作者单位： | 1. 华中科技大学同济医学院附属同济医院妇产科,武汉,430030;Department of Gynecology and Obstetrics, University Kiel, Germany 2. 华中科技大学同济医学院附属同济医院妇产科,武汉,430030 3. Department of Gynecology and Obstetrics, University Kiel, Germany |
| 摘 要： | 目的 :建立采用第一极体植入前染色体非整倍体诊断的方法。方法 :取试管婴儿后的未受精卵细胞 ,激光打孔法行第一极体活检 ,固定后行多色荧光原位杂交 (FISH) ,分析极体中 13,16 ,18,2 1和 2 2号染色体的核型情况。结果 :活检成功率为 94 .7% ,FISH成功率为 86 .7% ,6 1.5 %的极体核型正常 ,38.5 %的极体为非整倍体。结论 :激光打孔结合FISH法是一种快速有效的极体植入前诊断方法 |
| 关 键 词： | 卵细胞 第一极体 染色体 人 非整倍性 植入前诊断 原位杂交 荧光 |
| 文章编号： | 1004-7379(2004)05-0333-03 |
| 修稿时间： | 2004年3月31日 |
The use of first polar bodies for preimplantation genetic diagnosis |
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| Chen Wen ,Zhu Guijin ,Mettler L ..The use of first polar bodies for preimplantation genetic diagnosis[J].Current Advances In Obstetrics and Gynecology,2004,13(5):333-335. | |
| Authors: | Chen Wen Zhu Guijin Mettler L |
| Institution: | Chen Wen 1,2,Zhu Guijin 1,Mettler L 2.1.Department of Gynecology and Obstetrics,Tongji Hospital of Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030 China,2.Department of Gynecology and Obstetrics,University Kiel,Germany |
| Abstract: | Objective:To establish the method of preimplantation genetic diagnosis(PGD) by use of first polar bodies.Methods:The first polar bodies were taken out from unfertilized oocytes after IVF by laser assisted biopsy method. After fixation, chromosome 13,16,18,21 and 22 were analyzed in the first polar bodies by fluorescence in situ hybridization (FISH).Results:The successful biopsy and FISH rate were 94.7% and 86.7% respectively. 61.5% of the first polar bodies was found to be normal, 38.5% was aneuploidy.Conclusion:Laser assisted biopsy combined with FISH is a quick and effective method to detect aneuploidies in first polar bodies. |
| Keywords: | Oocytes First polar body Chromosomes human Aneuploidy Preimplantation genetic diagnosis In situ hybridization fluorescence |
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