三氧化二砷对人膀胱癌细胞BIU-87作用的体外研究

目的观察三氧化二砷(arsenic trioxide,As2O3)对人膀胱癌细胞株BIU-87的作用,并探讨其作用机制.方法采用四氮唑蓝(MTT)法检测As2O3不同浓度、不同时间对BIU-87细胞的生长抑制率,同时流式细胞仪检测不同浓度As2O3作用72 h后细胞中与凋亡有关蛋白Fas、Bcl-2的表达.结果As2O3可有效抑制BIU-87细胞的生长,具有浓度、时间依赖性特点.Fas、Bcl-2的表达分别与浓度增加呈正、负相关,P<0.05,且二者随浓度增高呈负相关,P<0.05.结论As2O3可有效抑制膀胱癌细胞生长,其作用机制与凋亡有关.

Experimental Study of Human Bladder Cancer Cell Line BIU-87 Treated by Arsenic Trioxide in Vitro

Objective To observe the effects of arsenic trioxide on human bladder cancer cell BIU 87 and study its mechanisms.Methods The growth inhibition rates of BIU 87 cell were investigated by MTT method with various concentration As 2O 3 and various time.Fas and Bcl 2 expressions were detected by flow cytometry after 72 hours with various concentration As 2O 3.Results As 2O 3 effectively inhibited the growth of BIU 87 cell,which depended on concentration of the drug and reaction time.Fas expression was positive correlation with the increasing concentration and Bcl 2 expression was negative so, P <0 05.It was negative correlation with the increasing concentration between Fas and Bcl 2 expressions, P <0 05.Conclusion As 2O 3 could significantly inhibit the growth of bladder cancer cells.Inducing cell apoptosis might be one of its action mechanisms.