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正交实验优选一贯煎多糖的提取工艺
引用本文:魏学军,林先燕,杨胜文,冯光维,李江.正交实验优选一贯煎多糖的提取工艺[J].辽宁中医药大学学报,2010(2).
作者姓名:魏学军  林先燕  杨胜文  冯光维  李江
作者单位:黔南民族医学高等专科学校;贵阳中医学院;
基金项目:黔南民族医学高等专科学校科研基金资助项目(QNYZ200803)
摘    要:目的:确定一贯煎中提取多糖的最佳工艺。方法:采用正交设计法,以加入水量(A)、提取时间(B)、提取次数(C)和醇沉浓度(D)作为因素。结果:C因素和D因素对多糖含量的影响具有显著意义,A因素、B因素的影响不显著。结论:最佳提取工艺为A1B2C2D2,即提取2次,时间分别为60min,加水量为8倍,醇沉浓度为70%。

关 键 词:一贯煎  多糖  正交试验  提取工艺  

Study on Extracting Craft of Polysaccharides in Yiguanjian
WEI Xue-jun,LIN Xian-yan,YANG Sheng-wen,FENG Guang-wei,LI Jiang.Study on Extracting Craft of Polysaccharides in Yiguanjian[J].Journal of Liaoning University of Traditional Chinese Medicine,2010(2).
Authors:WEI Xue-jun  LIN Xian-yan  YANG Sheng-wen  FENG Guang-wei  LI Jiang
Institution:1.Qiannan Medical College for Nationalities/a>;Duyun 558003/a>;Guizhou/a>;China/a>;2.Guiyang College of TCM/a>;Guiyang 550002/a>;China
Abstract:Objective:To fix optimum extracting craft of polysaccharides in Yiguanjian. Methods:The orthogonal design was used and water totaling (A),refluxing time (B),extraction time (C) and were defined(D)as factors of the design. Results:C factor and D factor had notable influence on the content of polysaccharides. Conclusion:Optimum extracting craft was defined as follows:A1B2C2D2.
Keywords:Yiguanjian  polysaccharide  orthogonal test  extracting craft  
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