紫杉醇抑制Hut78细胞生长及诱导凋亡的实验研究

目的：研究紫杉醇对皮肤T细胞淋巴瘤（cutaneous T cell lymphoma，CTCL）Hut78细胞株的生长抑制及诱导凋亡作用，并探讨其诱导凋亡作用的机制。方法：将紫杉醇按不同浓度、不同作用时间分别处理Hut78细胞，四甲基偶氮唑蓝（MTT）法测定Hut78细胞的生长抑制率，端粒酶重复序列扩增及酶联免疫吸附法（TRAP—PCR—ELISA）检测端粒酶活性的变化，采用形态学观察和流式细胞仪检测其诱导Hut78细胞凋亡的情况。结果：紫杉醇明显下调端粒酶活性表达，对Hut78细胞具有生长抑制及诱导凋亡的作用，且呈时间依赖和剂量依赖性。表现为G2／M期阻滞，出现典型的凋亡细胞特征，流式细胞仪检测可见明显的凋亡峰。结论：紫杉醇能下调端粒酶的活性，诱导细胞凋亡是其发挥抗癌作用的机制之一。

Effects of paclitaxel on the growth and apoptosis induction in Hut78 cell line

Objective: To observe the effects of paclitaxel on apoptosis induction in cutaneous T-cell lymphoma cell line Hut78 in vitro. Methods: Hut78 was treated by paclitaxel with different concentration and acting time. Telomerase activity was determined by TRAP-PCR-ELISA. The inhibition of Hut78 cell growth was measured by MTT assay. The apoptosis of Hut78 was examined by flow cytometry. Results: Paclitaxel could down-regulate telomerase activity, inhibit the growth of Hut78, and induce cell apoptosis in a dose and time dependent manner. S phase and G2/M phase DNA content increased and a typical subdiploid peak before G0/G1 phase were observed by flow cytometry. Conclusions: Paclitaxel has obvious effects of growth-inhibiting on cutaneous T-cell lymphoma and can induce apoptosis of Hut78 cell.