β-淀粉样蛋白对体外培养的神经干细胞作用研究

目的：观察β-淀粉样蛋白(Aβ)对体外培养的大鼠神经干细胞的作用。方法：取E13 d的Wistar胚胎大鼠脑组织，体外进行培养、传代、鉴定后，加入β-淀粉样蛋白，利用台盼兰拒染法细胞计数及MTT法观察细胞增殖及细胞活力，利用流式细胞术观察凋亡程度，同时观察加入β-淀粉样蛋白后，分化状态神经干细胞（加入10％胎牛血清）的变化。结果：当Aβ浓度大于25μmol/L时，能明显抑制神经干细胞的增殖及活力，同时引起神经干细胞的明显凋亡。而在12.5 μmol/L时就能抑制分化状态神经干细胞突起的延伸。结论：Aβ抑制神经干细胞的增殖分化并可致神经干细胞凋亡，可能是Alzheimer’s病的发病原因之一。

Effects of β- amyloid protein on cultured neural stem cells in vitro

AIM: To study the effects of β-amyloid protein (Aβ) on neural stem cells cultured in vitro. METHODS: Neural stem cells (NSC) were isolated from E13 SD rats and cultured in serum-free medium （DMEM/F12）. After detected by nestin, the Aβ was added to the NSC medium to observe the viability and proliferation of NSC by MTT, cell count and flow-cytometric examination. The effects of Aβ on differentiated NSC were also observed. RESULTS: Aβ markedly inhibited the proliferation and the cell viability of NSC when its concentration was higher than 25 μmol/L. The differentiatory ability of NSC was inhibited when Aβ was in very low concentration. CONCLUSION: Aβ significantly inhibits the proliferation and differentiation of NSC and this may be one of the reasons that Alzheimer’s disease is induced.