| 更昔洛韦的体外抗乙肝病毒活性 |
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| 引用本文： | 黄砚青,龚作炯,蔡鸿生,罗顺德. 更昔洛韦的体外抗乙肝病毒活性[J]. 中国医院药学杂志, 2000, 20(10): 589-592 |
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| 作者姓名： | 黄砚青 龚作炯 蔡鸿生 罗顺德 |
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| 作者单位： | 湖北医科大学附属第一医院,湖北武汉430060 |
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| 摘 要： | 目的：评价更昔洛韦（ＧＣＶ）体外抗乙肝病毒（ＨＢＶ）活性。方法：应用ＨＢＶ ＤＮＡ转染的人肝癌细胞株（ＨｅｐＧ２２．２．１５），进行ＧＣＶ体外抗ＨＢＶ活性的研究。结果：ＧＣＶ能明显减少ＨｅｐＧ２２．２．１５细胞培养上清液中ＨＢｓＡｇ、ＨＢＶ ＤＮＡ的产生。Ｓｏｕｔｈｅｒｎ印迹法分析：ＧＣＶ能显著抑制ＨＢＶ复制中间体（包括共价闭合环状态ＤＮＡ），而同时该药物对细胞形态、细胞计数及总细胞ＤＮＡ无明显的
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| 关 键 词： | 更昔洛韦 HepG22.2.15细胞 HBV |
| 文章编号： | 1001-5213(2000)10-0589-04 |
| 修稿时间： | 2000-01-13 |
Anti-hepatitis B virus effect of gancicloriv(GCV) in vitro |
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| HUANG Yan Qing,GONG Zuo Jiong,CAI Hong Sheng,et al. Anti-hepatitis B virus effect of gancicloriv(GCV) in vitro[J]. Chinese Journal of Hospital Pharmacy, 2000, 20(10): 589-592 |
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| Authors: | HUANG Yan Qing GONG Zuo Jiong CAI Hong Sheng et al |
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| Abstract: | OBJECTIVE:To study the inhibitory effect of gancilovir(GCV) on hepatitis B virus(HBV) replication in vitro .METHODS:To evaluate inhibitory effect of GCV with HBV transfected HepG 2 2.2.15 cell line in vitro .RESULTS:GCV could remarkably reduce the secret of HBsAg and HBV DNA in HepG 2 2.2.15 cell culture medium.Southern blot analysis demonstrated that GCV could remarkably inhibit HBV replicative intermediates including covalentely closed circular DNA. GCV and not significantly affect to cell morphology,cell counting and total cellular DNA.These results indicated that GCV can inhibit HBV replication in vitro ;meanwhile,the toxic effect to cells is not significant.CONCLUSIONS:GCV,as a novel,broad spectrum nucleotide analogue has anti HBV activity,can be used further in clinical study. [ |
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| Keywords: | GCV hepatitis B virus HepG_2 2.2.15 cell |
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