紫心甘薯黄酮改善L6细胞胰岛素抵抗的有效部位筛选

摘 要 目的：采用L6细胞胰岛素抵抗模型，筛选出能有效改善胰岛素抵抗的不同极性紫心甘薯黄酮提取液，并初步探讨其作用机制。方法: 建立L6细胞胰岛素抵抗模型，采用不同极性的紫心甘薯黄酮提取液进行干预，观察并比较加液前后的细胞胰岛素抵抗L6细胞的剩余葡萄糖量。结果: 紫心甘薯总黄酮提取液、正丁醇提取液中、高剂量组，氯仿提取液低、中、高剂量组葡萄糖剩余浓度明显小于IR组（P<0.05）。结论:紫心甘薯总黄酮提取物、氯仿萃取液、正丁醇萃取液均具有缓解L6细胞胰岛素抵抗的作用。

Screening of Active Fractions for Purple Sweet Potato Flavonoids with Insulin Resistance Improving Ability of L6 Cells

ABSTRACT Objective: To screen the active fractions from purple sweet potatoes containing flavonoids with insulin resistance improving ability. Methods: L6 cell model with insulin resistance was established. The extracting solutions with different polarity of purple sweet potato flavonoids were used to affect the model. The residual glucose concentration in insulin resistant L6 cells was observed and compared before and after the intervention. Results:The residual glucose concentration of total flavonoids extracting solution, butanol extracting solution at middle and high dose, and chloroform extracting solution at all doses of purple sweet potato were lower than that of IR group (P<0.05). Conclusion:The total flavonoids extract, chloroform extracting solution and butanol extracting solution of purple sweet potato can improve insulin resistance in L6 cells.