大黄对急性出血性卒中大鼠中枢神经二次损伤纤溶机制的影响

目的 观察大黄对急性出血性卒中大鼠中枢神经二次损伤现象的治疗作用，并探讨其可能作用机理．方法自体血脑内注入造成大鼠急性出血性卒中模型，观察大黄对模型大鼠中枢神经二次损伤以及脑内纤溶酶原(PLG)和组织型纤溶酶原激活物(tPA)的影响。结果 (1)大约70％模型大鼠于造模后4～6天再次出现神经功能缺损体征，第4天评分为(1．63±0．72)分，中枢神经二次损伤于造模后6～8天达到高峰，第7天评分为(2．32±1．12)分。(2)大黄能有效改善这种神经功能二次损伤现象、自用药后第4天起，大黄组大鼠神经功能缺损评分一直维持在(1．24±0．19)分左右，未出现明显中枢神经二次损伤现象第7天大黄组大鼠神经功能缺损评分为(1．22±0．15)分，与模型组第7天比较，差异有显著性（P<0．05)(3)模型组大鼠第3天中枢tPA mRNA特异扩增产物及第7天中枢PLG mRNA特异扩增产物均明显高于假手术组；大黄组tPA mRNA、PLG mRNA表达明显低于模型组。结论 大黄能有效治疗急性出血性卒中大鼠中枢神经二次损伤，这一作用与大黄抑制急性出血性卒中大鼠中枢tPA mRNA及PLG mRNA表达有关。

Effect and Mechanism of Rhubarb on Fibrinolysis in Secondary Damaged Central Nerve System of Rats with Acute Hemorrhagic Stroke

OBJECTIVE: To observe the effect of rhubarb in treating secondary damage of central nerve system (CNS) in rats with acute hemorrhagic stroke (AHS) and to explore the possible mechanism. METHODS: The rat's AHS model was established by autologous blood injection, the effect of rhubarb on the secondary damage of CNS, plasminogen (PLG) in brain and tissue type plasminogen activator (t-PA) were observed. RESULTS: (1) The nerve function deficit signs reappeared in about 70% model rats 4 - 6 days after modeling and reached the peak at day 6 - 8, scored as 1.63 +/- 0.72 on day 4 and as 2.32 +/- 1.12 on day 7; (2) Rhubarb could effectively improve the secondary nerve function damage, with the nerve deficit scores kept to 1.24 +/- 0.19 from day 4 on, and no sign of secondary CNS damage was shown. The nerve deficit score was 1.22 +/- 0.15 on day 7 in the rhubarb treated group, showing significant difference as compared with that in the model group (P<0.05); (3) The specific amplified products of t-PA mRNA on day 3 and that of PLG mRNA on day 7 in CNS of model group were significantly higher than those in the sham operated group and the rhubarb treated group. CONCLUSION: Rhubarb could effectively reduce the secondary CNS damage in rats with AHS, it might be related with the suppressive effect of rhubarb on tPA mRNA and PLG mRNA expression in CNS.