Hp感染与老年胃癌及癌前病变中bcl-2、p16、c-myc基因表达的关系

目的　研究幽门螺杆菌 (Hp)感染的老年胃癌及癌前病变 bcl- 2、 p1 6、 c- myc基因蛋白的表达。方法　 bcl- 2、 p1 6、 c- myc蛋白检测采用免疫组化方法 ,快速尿素酶法和 HE染色确定 Hp感染。结果　 c- myc在胃癌中阳性表达率显著高于异型增生 (P<0 .0 5)。 bcl- 2在异型增生组织中的阳性表达率与胃癌、肠上皮化生相比无显著性差异 (P>0 .0 5)。bcl- 2与胃癌类型、分化程度显著相关 (P<0 .0 5)。Hp阳性组 bcl- 2、c-myc阳性表达率均高于 Hp阴性组 ,两者之间有显著性差异 (P<0 .0 5)。 p1 6在慢性胃炎中阳性表达率显著高于胃癌 (P<0 .0 5)。 Hp阳性萎缩性胃炎 p1 6阳性表达率低于 Hp阴性组 (P<0 .0 5)。结论　 Hp感染可以引起抑癌基因 p1 6表达低下 ,c- myc、bcl- 2基因蛋白表达增加 ,Hp可能为促癌剂在胃癌的发生发展中起一定作用。

Investigating the Relationship of Hp Infection to Gastic Cancer and the Expression of bcl-2, p16 Gene, c-myc of precancer in old people

Objectives To investigate the possible role of Helicobacter pylori (Hp) infection the development of gastric cancer and the increased expression of bcl 2, c myc and the decreased of precancer in old people. Method Immunohistochemical staining was used to detect the expression of p16, bcl 2, c myc protein. Hp was assessed by rapid urease staining test combined with HE staining test. Results The positive rate of bcl 2 was no correlation between gastric carcinoma, intestinal metaplasia and dysplasia ( P >0.05), but significantly in gastritis ( P <0.05). Its expression level was intensive in Hp positive patients when compared with Hp negative patients in precancerous lesions and carcinoma ( P <0.05). The positive rate of bcl 2, c myc protein in gastric carcinoma was significantly higher than that in dysplasia ( P <0.05), especially in Hp positive patients. The positive rate of p16 expression in patient with chronic atrophic gastritis was higher than gastric cancer and Hp positive was lower than those with Hp negative ( P <0.05). Conclusions Hp infection may induce p16, bcl 2, c myc gene expression alteration occurred in gastric cancer. Hp might act as a tumor promoter in the genesis of gastric carcinoma.