某市饮用水原水、自来水及经不同浓度活性炭处理后自来水对大鼠原代肝细胞DNA损伤修复的影响研究

为研究不同浊度的原水、自来水、加入不同浓度的活性炭处理后的自来水水样有机提取物的致突变性 ,进行了大鼠肝细胞程序外DNA合成 (UDS)试验研究。结果表明 :自来水、加活性炭 5、10、15mg L处理组以及各种浊度的原水水样有机提取物UDS试验均呈阳性结果 ,且每组随加入水样体积增高而出现CPM值上升。水样致突变性随浊度升高而增强 ,自来水致突变性高于原水 ,自来水加活性炭 2 0mg L处理后未检出程序外DNA合成。通过加强混凝沉淀、过滤和活性炭吸附等常规水处理工艺 ,可以降低或消除自来水的致突变性

Study on the unscheduled DNA synthesis in primary rat hepatocyte induced by source water, tap water and active carbon-treated tap water

To study the mutagenicity of source water with different turbidities, tap water and active carbon treated tap water,unscheduled DNA synthesis in primary rat hepatocyte was detected. Increased unscheduled DNA synthesis was found in a dose depend manner inducted by tap water, source water and tap water treated with 5, 10 and 15 milligrames active carbon per liter water. The results also showed that the mutagenicity of the source water increased with the increasing of its turbidity, and mutagenicity of the tap water is higher than that of the source water as well. There was no unscheduled DNA synthesis in tap water followed by the treatment with 20 milligrames active carbon per liter water. It is suggested that the development of the water treatment technics including sedimentation, filtration and treatment with active carbon is needed for decreasing or eliminating the mutagenicity of tap water.