局灶性脑缺血时吡拉西坦对缺血半球能量代谢的影响

目的　观察吡拉西坦对局灶性脑缺血时缺血半球能量代谢的影响。方法　采用动脉腔内插线大鼠局灶性脑缺血模型及测定缺血 2 h/再灌注 2 h后缺血半球内乳酸 ( L A)水平、乳酸脱氢酶 ( L DH)和肌酸激酶 ( CK)活性的变化 ,HE染色结合图像分析测定缺血后 2 4h的梗死体积。结果　吡拉西坦 ( 2 0 0 mg/kg)可明显降低缺血半球内 L A水平 ,增加 L DH的活性 ,对 CK活性无明显影响 ,它可明显降低缺血 2 h/再灌注 2 2 h后的皮质、皮质下结构和半球的梗死体积。结论　吡拉西坦具有明确的抗局灶性脑缺血作用 ,它可明显改善缺血组织的能量代谢状态 ,增加 L DH的活性 ,减轻乳酸堆积及组织酸中毒 ,缩小梗死体积。

The effects of piracetam on the energy metabolism in ischemic hemisphere in the rat model of focal cerebral ischemia

Objective To observe the effects of piracetam on the energy metabolism in the model of focal cerebral ischemia.Methods The rat model of focal cerebral ischemia induced by occlusion of intraluminal filament was used.The level of lactic acid(LA) and activities of lactate dehydrogenase(LDH) and creatine kinase(CK) in ischemic hemisphere after 2h of ischemia and 2h of reperfusion were determined by biochemical analysis and the infarct volume after 24h of ischemia was measured by HE staining combined with image analysis.Results Piracetam(200mg/kg) decreased the level of LA and enhanced the activity of LDH in ischemic hemisphere caused by focal cerebral ischemia,and had no effect on the activity of CK.It reduced the infarct volume after 24h of ischemia significantly.Conclusion Piracetam may improve the energy metabolism and attenuate ischemic injury caused by focal cerebral ischemia.