诱生型一氧化氮合酶抑制剂氨基胍对鼠胚发育的影响

目的：研究诱生型一氧化氮合酶（inducible nitric oxide synthase ，iNOS）选择性抑制剂氨基胍（aminoguanidine, AG）对鼠胚发育的影响，以探讨iNOS对妊娠的作用机制。方法：雌性昆明小鼠，自妊娠第3、7、14天开始皮下注射AG 10或20 mg*kg－1*d－1，直到妊娠第19天取材，测量鼠胎、胎盘重量，记录胚胎数、吸收点，并观察胎盘、脐带组织学变化，用免疫组化、还原型烟酰胺腺嘌呤二核苷酸磷酸黄递酶组化方法测量胎盘脐带内的NOS活性。结果：早期子宫孕珠有吸收的表现。孕早期、中期用药组母鼠重量、胚胎数减少，吸收点增多，但不影响鼠胎、胎盘的重量。图像分析胎盘内iNOS表达量差异无显著性。结论：AG抑制小鼠妊娠早、中期胚胎的着床与发育，但对已经成形的胚胎及胎盘影响不明显。

The effect of an inhibitor of inducible nitric oxide synthase on the embryo development of mice

Objective: To determine whether aminoguaidine（AG）,a selective inhibitor of inducible nitric oxide synthase (NOS), affect the embroy development of mice, and to study the mechanisms of iNOS affecting on pregnancy. Methods: AG(10 mg*kg－1*d－1 or 20 mg*kg－1*d－1) were injected subcutaneously to mice from day 3，7，14. kill mice at day19. The weight of fetus and placenta, the number of fetus and fetal resorptions were measured. Histological changes of placenta and umbilical cord were observed. Immunohistochemistry and NADPH histochemistry were adopted to study NOS activity of placental and umbilical cord respectively. Results: The conceptus in the uterus were resorbed in the early pregnancy. In the early and middle pregnancy the weight of pregnant mouse and the number of fetus of AG groups was decreased. The number of fetal resorptions was increased. But the weight of fetus and placenta were not affected. The expression of iNOS in the placenta were no difference significantly between the two groups with image analysis. Conclusion: AG inhibits the implantation and growth of embryo in the early and middle pregnancy, buy doesn't affect the formed fetus and placenta significantly.