nm23-H1及热休克蛋白27在非小细胞肺癌中的表达及临床意义

 目的　分析nm23-H1和热休克蛋白27（HSP27）在非小细胞肺癌（NSCLC）发生、发展及转移中的作用。方法　应用免疫组织化学法检测75例NSCLC组织及28例良性肺病变中nm23-H1和HSP27的表达情况，并将检测结果与临床病理特征进行综合分析。结果　nm23-H1和HSP27的表达主要定位于细胞质，nm23-H1和HSP27在NSCLC中表达的阳性率分别为41.3 %（31／75）和80.0 %（60／75），明显高于良性肺病变7.1 %（2／28）和46.4 %（13／28）（χ2＝10.946，P＝0.001；χ2＝11.131，P＝0.001）。NSCLC中HSP27蛋白的表达与肿瘤分化程度密切相关（χ2＝4.191，P＝0.041）。肺癌组织中nm23-H1、HSP27 的表达水平有相关性（r＝0.284，P＝0.013）。结论　nm23-H1、HSP27与NSCLC的发生、发展有关，二者联合检测对肺癌的诊断和生物学行为判断有一定的意义。

Expression of nm23-H1 and heat shock protein 27 and their significance in non-small cell lung carcinoma

Objective　To detect the expressions of nm23-H1 and heat shock protein 27 (HSP27) and their clinical significance on development and metastasis in non-small cell lung carcinoma (NSCLC). Methods　75 tumor tissues from patients with NSCLC were included as experimental group and 28 pulmonary benign lesion tissues were as control group. The expressions of nm23-H1 and HSP27 in patients with different clinical and pathological characters were detected by immunohistochemistry. Results　nm23-H1 and HSP27 were mainly expressed in cytoplasm, the positive rates of nm23-H1 and HSP27 were significantly higher in the experimental group than that in control group [41.3 % (31/75) vs 7.1 % (2/28), χ2 = 10.946, P = 0.001, 80.0 % (60/75) vs 46.4 % (13/28), χ2 = 11.131, P = 0.001]. Compared with control group, the positive rate of HSP27 was correlated with the degree of tumor differentiation (χ2 = 4.191, P = 0.041). nm23-H1 was related with HSP27 in lung cancer (r = 0.284, P = 0.013). Conclusion　nm23-H1 and HSP27 are related to the occurrence and development of NSCLC. The joint detection of nm23-H1 and HSP27 should be helpful to the diagnosis and judge the biological behavior of NSCLC.