雌二醇及雌酮对人子宫内膜细胞GJIC及连接蛋白的影响

【目的】 探讨雌二醇(E2)及雌酮(E1)对体外培养的人子宫内膜腺上皮细胞及间质细胞间隙连接细胞间通讯(GJIC)及连接蛋白(Cx)43?32表达的影响?【方法】 采用划痕染料标记示踪技术及激光共聚焦显微镜扫描技术(LSCM),观察E2及E1对原代培养的20例人子宫内膜腺上皮细胞及间质细胞GJIC的影响,采用激光共聚焦显微镜扫描技术,观察E2及E1对Cx 43?Cx 32表达的影响?【结果】 5 × 10-5 mol/L雌二醇作用48 h后间质细胞及腺上皮细胞GJIC功能没有明显变化(P > 0.05),2.5 × 10-5 mol/L雌酮作用间质及腺上皮细胞48 h后,细胞GJIC功能有所下降(P 0.05)?【结论】 E1抑制细胞GJIC功能,提示其在子宫内膜增殖性病变的阶段扮演重要角色;E1及E2均未影响子宫内膜细胞Cx43?Cx32的表达,提示Cx表达量不能完全反映GJIC功能?

Effect of Estradiol-17 Beta and Estrone on Gap Junction Intercellular Communication and Connexin of Human Endometrial Cell

【Objective】 To explore the effects of estradiol-17 beta and estrone on gap junction intercellular communication and Cx43 and Cx32 expression of stromal and glandular cells in vitro. 【Methods】 The scrap loading dye transfer was used to investigate the effects of E1 and E2 on GJIC of endometrial stromal and glandular cells. The influence of E1 and E2 on Cx43 and Cx32 expressions was investigated with a laser scanning confocal microscope. 【Results】 The ability of GJIC function was higher in the endometrial stromal cells than that of the glandular cells in vitro. 2.5 × 10-5 mol/L E1 leading to smaller GJIC after 48 h(P < 0.05). Cx32 expression in endometrial stromal and glandular cells were significantly higher than Cx43. 5 × 10-5 mol/L E2 and 2.5 × 10-5 mol/L E1 had no effect on Cx expression. 【Conclusions】 The function of Estrone’s down-regulation on GJIC of endometrial stromal and glandular cells may be one of mechanisms which invert endometrial hyperplasia. E1 and E2 had no effect on Cx expression of endometrial stromal and glandular cells, which indicate Cx expression could not completely reflect GJIC function in vitro.