5-氟尿嘧啶对口腔鳞癌细胞株热休克蛋白27／70表达的影响

目的：为探讨热休克蛋白27和热休克蛋白70在口腔鳞状细胞癌化疗中的意义。本实验选用口腔鳞状细胞癌细胞株OSC-4作为研究对象t随机分组。实验组加100μg／mL 5-氟尿嘧啶。方法：用MTT法检测细胞存活率，流式细胞仪检测细胞凋亡，Western Blot检洲HSP27和HSP70的表达。结果：OSC-4细胞经5-Fu处理后，MTT和流式细胞仪显示5-Fu抑制OSC-4细胞的存活率（P〈0．05）．并存在时间和剂量依赖。Western Blot结果显示HSP27和HSP70在OSC-4中均有表达，在OSC-4细胞中5-Fu能诱导HSP27和HSP70的表达（P〈0．05）。HSP27和HSP70在不同口腔鳞状细胞癌细胞株中均有表达，表达有差异（P〈0．05）。结论：5-Fu能诱导OSC-4细胞的凋亡，并呈现时间与剂量依赖；5-Fu能上调HSP27和HSP70的表达。

Effects of 5-Fluride on heat shock protein 27 and 70 in oral squamous carcer cell line

Purpose： To study the significance of HSP27 and HSP70 in chemotherapy for oral squamous carcer （OSC）. In this experiment, OSC cell line 4 （OSC--4） was selected for sample,which was separated at random and cultured in DMEM medium with 100 μg/mL 5--Fu. Methods：We used MTT to assay the survival of OSC--4 cell, the flow cytometry to detect apoptosis and Western Blot to detect the levels of HSP27 and HSP70. Results：The survival OSC--4 cell was inhibited after the treatment with 5--Fu （P 〈 0.05） . Western Blot showed the HSP27 and HSP70 expression in OSC--4,which could be enhanced by 5--Fu. Besides, there was the expression of HSP27 and HSP70 in other OSC cell lines,but the latter expression was different from the former expression. Conclusion： 5--Fu induces the apoptotic damage,showing the time and dose dependence in OSC--4 and up--regulates the expression of HSP27 and HSP70.