早期应用神经营养因子对周围神经损伤修复的研究观察

[目的]研究探索神经生长因子(nervegrowth factor NGF)、碱性成纤维细胞生长因子(basic fibroblast growth factor bFGF)联用对大鼠坐骨神经钳夹损伤后神经纤维再生修复的促进作用和对失神经支配的小腿三头肌的保护作用。[方法]手术钳夹损伤坐骨神经后，损伤处神经内注射NGF和bFGF，对照组注射等量生理盐水，术后每日在术侧腓肠肌内注射药物，术后4、8、12周，进行足印分析及坐骨神经功能指数测定，取小腿三头肌称湿重，取脊髓及神经肌肉行组织病理观察。[结果]联用NGF bFGF，能显著改善神经损伤后引起的神经功能指数，有效地减轻由于失神经支配后肌肉的萎缩，促进神经纤维的修复与再生。[结论]联用NGF、bFGF对大鼠坐骨神经钳夹损伤后神经纤维再生修复具有显著的促进作用，可减轻失神经支配的小腿三头肌的萎缩。

Early - using effect of nerve growth factor and basic fibroblast growth factor on injured peripheral nerve

[Objective]To explore the improving effect of nerve growth factor (NGF) and basic fibroblast growth factor (bFGF) on neural fiber's regeneration after injuring sciatic nerve by clamping compression, and to investigate the protective effect on the denervated sural triceps in rats.[Method] In the experimental group, NGF and bFGF were injected to nerves which were injured by clamping; in the control group, equal amount of saline was injected. Drugs were injected into sural triceps once everyday for a month after this operation. In the 4th, 8th and 12th week, foot analyses were performed respectively. Pathological observation of the spinal cord, sciatic nerve and sural triceps were conducted after applying anesthesia and perfusion to the animal.[Result]The combining application of NGF and bFGF significantly improved the function index of the injured nerve, reduced the denervated muscle's atrophy and increase the nerve fiber's regeneration.[Conclusion]The combining application of NGF and bFGF can significantly improve the repairing and regeneration effect of clamped sciatic nerve and decrease the atrophy of denervated sural triceps.