Angiopoietin-2和VEGF在口腔鳞癌中的表达及意义

目的研究口腔鳞癌组织中Ang-2和VEGF的表达并分析它们与肿瘤临床病理学特征和血管生成及血管成熟间的关系。方法:用常规的免疫组织化学的方法检测41例口腔鳞癌及30例癌旁正常组织和10例正常口腔黏膜中的Ang-2及VEGF的表达;通过双标免疫组织化学法同时染CD34(标记所有血管内皮细胞)和α-平滑肌肌动蛋白(标记血管壁细胞包括血管平滑肌细胞和周细胞)评估微血管密度(MVD)及血管成熟指数(VMI)。结果在口腔鳞癌组织中Ang-2和VEGF的阳性表达率分别为21(51.22%)和26(63.42%);Ang-2和VEGF在口腔鳞癌组织中表达显著高于它们在癌旁正常组织(P<0.05)和正常口腔黏膜组织中的表达(P<0.05);Ang-2表达与肿瘤的淋巴结转移密切相关(P<0.01)而VEGF表达与肿瘤的肿瘤分化程度相关(P<0.05),它们与病人的性别、年龄及TNM分期无关(P>0.05);Ang-2和VEGF表达阳性的鳞癌组织MVD显著高于它们阴性表达组(P<0.05)而Ang-2表达阳性的鳞癌组织VMI显著低于Ang-2表达阴性组(P<0.05)。在联合VEGF表达的情况下,同时表达Ang-2和VEGF的肿瘤MVD(51.08±2.99)显著高于其他任何表达状况(P<0.05)。结论Ang-2和VEGF在口腔鳞癌组织中的过表达可能在口腔鳞癌的进展过程中起重要作用;它们与肿瘤的血管生成和成熟密切有关。

Expression of Angiopoietin-2 and Vascular Endothelial GrowthFactor in Oral Squamous Cell Carcinoma and Its Significance

Objective: To investigate the expression of angiopoietin-2 (Ang-2) and vascular endothelialcellgrowth factor (VEGF) in oral squamous cell carcinoma (OSCC) and their correlations with clinicopathologicparameters, angiogenesis and vessel maturation of OSCC. Methods: The expression of Ang-2 andVEGF was detected in 41 speciments of human OSCC, 30 adjacent noncancerous oral tissues and 10specimens of normal oral mucosa by conventional immumohistochemistry. Microvessel density (MVD) andvessel maturation index (VMI) were also assessed by double-labelling immumohistochemistry stainingagainst CD34, a marker of pan-endothelial cells, and that against alpha-smooth muscle actin (α-SMA),a marker of mural cells (pericytes/smooth muscle cells). Results: The positive expression rate of Ang-2and VEGF in 41 OSCC tissues was 51.22% and 63.42%, respectively. The expression of Ang-2 and VEGFwas significantly higher in OSCC than in adjacent noncancerous oral tissues (all P<0.05) and normal oralmucosa (all P<0.05). In the clinicopathologic parameters, the Ang-2 expression was closely correlated withtumor lymph node metastasis (P<0.01) and the VEGF expression was correlated with tumor differentiateddegree (P<0.05), but there was no significant correlation among the Ang-2 and VEGF expressionand patients’ sex, age and TNM stages (all P>0.05). The MVD of OSCC positive for both Ang-2 andVEGF was significantly higher than that of OSCC negative for both Ang-2 and VEGF (P<0.05). TheVMI of OSCC positive for Ang-2 was significantly lower than that of OSCC negative for Ang-2 (P<0.05).When Ang-2 expression was combined with the staus of VEGF expression, MVD of OSCC positive forboth Ang-2 and VEGF was the highest (51.08±2.99) as compared with that of other status in patient withOSCC (all P<0.05). Conclusion: The overexpression of Ang-2 and VEGF may play a crucial role in thedevelopment of OSCC. They are closely associated with angiogenesis and vessel maturation of tumor.