| 野生型p53对胃癌细胞系的生长抑制作用 |
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| 引用本文： | 王丽君,邹卫,张乃衡.野生型p53对胃癌细胞系的生长抑制作用[J].北京大学学报(医学版),1997(1). |
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| 作者姓名： | 王丽君 邹卫 张乃衡 |
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| 作者单位： | 北京医科大学生物化学与分子生物学系!100083 |
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| 摘 要： | 目的：观察野生型p53对胃癌细胞系的生长抑制作用。方法：以逆转录病毒为载体将野生型p53基因导入胃癌细胞系MKN28和BGC823，检测p53对肿瘤细胞的影响。结果：p53在转染细胞中表达水平提高。外源性p53的导入使肿瘤细胞增殖细胞核抗原（PCNA）水平明显降低；G0／G1期细胞数增加，S期降低。转染p53基因的MKN28细胞探鼠体内成瘤能力明显下降。结论：野生型p53基因参与调节DNA复制及细胞周期，抑制癌细胞过度增殖。
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| 关 键 词： | 胃肿瘤／治疗 基因疗法 基因.p53 脱氧核糖核酸复制 |
THE GROWTH-SUPPRESSIVE EFFECT OF WILD-TYPE p53 ON GASTRIC CANCER CELL LINES |
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| WANG Lijun, ZOU Wei, ZHANG Naiheng.THE GROWTH-SUPPRESSIVE EFFECT OF WILD-TYPE p53 ON GASTRIC CANCER CELL LINES[J].Journal of Peking University:Health Sciences,1997(1). |
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| Authors: | WANG Lijun ZOU Wei ZHANG Naiheng |
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| Abstract: | Inhibitory effects of wild-type p53 on gastric cancer cell lines. Methods: Usingthe retroviral vector to introduce wild-type p53 into human gastric cancer cell line MKN28 and BGC823,The effects of p53 on these two tumor cell lines were identified. Results: Expression of p53 in transfected cell lines was increased. The introduction of exogenous wild-type p53 into tumor cells results in decreased level of proliferative cell nuclear antigen (PCNA), higher G0/G1 ratio and lower S ratio in cellcycle distribution in comparison with the control. The tumorigenicity fo MKN28 cells in nude mice wassuppressed by exogenous p53. Conclusion: p53 plays an important role in regulating DNA replicationand cell cycle, and in suppressing cell proliferation. |
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| Keywords: | Stomach neoplasms/ther Gene therapy Gene p53 DNA replication |
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