应用VIDAS免疫分析系统检测血清抗伯氏包柔螺旋体抗体

莱姆病（Ｌｙｍｅｄｉｓｅａｓｅ）是最近十年来才被认识的一种蜱媒传染流行病，Ｌｙｍｅ病的血清学诊断方法有间接荧光免疫法（ＩＦＡ），酶免疫试验（）ＥＩＡ），免疫印迹法（ＷＢ），梅里埃生物公司（Ｂｉｏｍｅｒｉｅｕｘ）推出ＶＩＤＡＳ免疫诊断系统能够自动定性和半定量地检查Ｌｙｍｅ病的抗伯氏包柔螺旋体抗（抗ＢＢ抗体）。本方法结合了酶和荧光免疫两种方法。硷性磷酸酶标记二抗的底物是４ｍｅｔｈｙｌｕｍｂｅｌｌｉｆｅ

Detection of Anti-BB Antibodies in Human Sera by VIDAS System

Lyme disease is an infectious disease just known in the latest decade. The sera of people from various origins were detected for Anti-BB antibodies (IgM+IgG) by VIDAS system. The results are as follows:Of 83 sera from the health-cared people, 8 were positive (9.6%); of 20 sera from the patients clinical diagnosised as tuberculosis, none was positive; of 46 sera from the patients clinical diagnosised or suspected as node diseasas, 4 were positive (8.7%); of 57 sera from the patients with various ophthalmologic diseases, 13 were positive (22.8%); of 15 sera from the patients with stomatologic diseases, 4 were positive (26.7%); of 17 sera from the patients with various diseases including dermomyositis, liver and spleen swelling, undefinited febrile diseases; lung cancer, lung shadow, etc.), 2 were positive (11.8%); of 16 sera from the people highly suspected with lyme disease living in the epidemic areas of Xin Jiang and Hei Long Jiang provinces, 12 were positive (75%).
The same 165 sera samples were detected at the same time by VIDAS System and IFA, 24 were positive by VIDAS System (14.5%), and 131 positive by IFA (79%), the accordance rate was only 32.7%.