应用系列呼吸道病毒单克隆抗体快速检测分泌物中的病毒抗原

应用系列主要时吸道病毒［呼吸道合胞病毒（ＲＳＶ），甲型流感（ＦｌｕＡ）、乙型流感（ＦｌｕＢ）、副流感１、３型（ＰＩＶ１．３）、副流感２型（ＰＩＶ２）病毒共６种］单克隆抗体（ＭｃＡｂ），联接ＡＰＡＡＰ桥联技术，深入部队对２６０例呼吸道感染青年战士的鼻咽部脱落细胞中的抗原进行检测。检出ＦｌｕＡ抗原１８例，ＰＩＶ２抗原１０例，ＦｌｕＢ和ＲＳＶ各１例。以上结果经间接免疫荧光（ＩＦＡ）以及阻断和替代试验，证实系列单克隆抗体检出抗原结果是特异的。笔者认为本方法是临床快速诊断以及流行病学调查的有效手段。

Rapid Detection for Antigens of Respiratory Tract Viruses by Using Serizes of Monoclonal Antibody

series of monoclonal Antibodies(McAb)against six main respiratory tract viruses(RTV) respiratory syncytial virus(RSV),InfluenzaA virus(FluA),influenza B vjrus(FluB),para-influenza virus type1.2.3(PIV1.2.3)were deve-loped,and were used in APAAP bridge-enzymeassay to rapidly detect the antigens of RTV.Total 260 samples of nasopharyngeal exfoliatedcells were collected from young men in BeijingArmy suffered from respiratory tract virus in-fections.The results showed that 18 cases weretested with FluA antigen;10 with PIV2 antigenand 1 with FluB and another with RSV antigen,respectively.All the positive samples were de-termined by indirect immunofluorescence assay(IFA) and blocking test and replacement test.It is suggested that the method used in the study be effective in elinical rapid diagnosisand epidemiological survey.