康艾注射液对人结直肠癌Lovo细胞Th1/Th2类细胞因子的影响 |
| |
引用本文: | 刘季芳,祁岩超,卢敏莹,潘东晓,申鸿卓. 康艾注射液对人结直肠癌Lovo细胞Th1/Th2类细胞因子的影响[J]. 国际医药卫生导报, 2009, 15(14). DOI: 10.3760/cma.j.issn.1007-1245.2009.14.008 |
| |
作者姓名: | 刘季芳 祁岩超 卢敏莹 潘东晓 申鸿卓 |
| |
作者单位: | 广州医学院附属肿瘤医院,510095 |
| |
摘 要: | 目的 探讨康艾注射液对人结直肠癌Lovo细胞Th1/Th2状态的影响.方法 常规培养Lovo细胞,待细胞处于指数增长期再分成4组:1组为空白对照,另外3组加入不同浓度的康艾注射液.培养72h后,采用双抗夹心酶联免疫吸附法(ELISA)分别检测各组Lovo细胞培养上清中Th1类细胞因子(IL-2,IFN-γ)和Th2类细胞因子(IL-4,IL-10)的水平.结果 Lovo细胞与康艾注射液共培养后,上清液中Th2类细胞因子的含量较对照组明显降低(P<0.05),而Th1类细胞因子在各组中的含量变化不大.结论康艾注射液可显著下调Lovo细胞Th2类细胞因子水平,具有促进其Th2向Th1逆转的倾向.
|
关 键 词: | 康艾注射液 逆转 免疫治疗 |
Effect of Kang'ai Injection on Th1/Th2 cytokines in human colon carcinoma cell line Lovo |
| |
Abstract: | Objective To explore the effect of Kang'ai injection on Th1/Th2 cytokine in human colon carcinoma cell line Lovo.Methods Lovo cells were cultured by conventional ways until they were in exponential growth phase and then divided into 4 groups:one control group and three experimental groups with different concentration of Kang'ai injection.After 72 hours culture,Th1 type cytokines (IL-2,IFN-γ) and Th2.type cytokines (IL-4,IL-10) in the culture supernatant of Lovo cells in each group were separately detected by double-antibody sandwich enzyme-linked immunosorhent assay (ELISA).Results After the coculture of Lovo cells and Kang'ai injection,Th2 type cytokine levels in the supernatants were significant lower than the control group (P<0.05),and Th1 type cytokines in each group were of little change.Conclusions Kang'ai injection markedly downregulated Th2 type cytokines in Lovo cells,and contributed to the switching from Th2 to Th1. |
| |
Keywords: | Th1/Th2 Kang'ai injection Th1/Th2 Reversion Immunotherapy |
本文献已被 万方数据 等数据库收录! |
|