支气管哮喘患者变应原检测及免疫分子研究

目的：了解支气管哮喘患者变应原检测情况，并研究支气管哮喘患者血清中 TNF-α，IL-4，IL-6及 IL-8的水平变化及临床意义。方法选择宝鸡市中心医院2011年8月～2013年8月期间收治的62例支气管哮喘患者作为观察组，采用酶联免疫吸附试验检测变应原。选取查体健康者62例作为对照组，测定两组血清中 TNF-α，IL-4，IL-6及 IL-8的水平。结果支气管哮喘患者最主要的过敏原是粉尘螨（58例，阳性率93.5％），其次是室内尘土，观察组血清 TNF-α水平高于对照组（230.87±27.82 ng／ml vs 152.14±23.27 ng／ml），差异有统计学意义（P＜0.05）；观察组血清中 IL-4，IL-6，IL-8水平明显高于对照组（25.32±12.04 ng／L vs 8.57±3.64 ng／L，152.45±24.56μg／L vs 98.56±18.63μg／L，218.79±32.91μg／L vs 142.68±23.76μg／L），差异均有统计学意义（P＜0.01）。结论 TNF-α，IL-4，IL-6及 IL-8水平可作为支气管哮喘患者判断的一个重要预测指标，值得临床推广应用。

Study on Allergen Detection and Immune Molecules in the Patients with Bronchial Asthma

Objective To investigate the allergens and study the clinical significance of detecting serum levels of TNF-α,IL-4, IL-6 and IL-8 in patients with bronchial asthma.Methods 62 cases of patients with bronchial asthma were selected from August 2011 to August 2013 in Baoji Central Hospital,enzyme-linked immunosorbent assay were carried out in patients u-sing standardized allergens.62 cases of healthy people were selected as control group,and detected serum levels of TNF-α, IL-4,IL-6 and IL-8 of the two groups.Results Dust mites were the most important and the strongest allergens.The levels of TNF-α,IL-4,IL-6 and IL-8 in observation group was significantly higher than those in control group （230.87±27.82 ng/ml vs 152.14± 23.27 ng/ml;25.32 ± 12.04 ng/L vs 8.57 ± 3.64 ng/L;152.45 ± 24.56 μg/L vs 98.56 ± 18.63 μg/L;218.79±32.91μg/L vs 142.68±23.76μg/L,P〈0.05）.Conclusion TNF-α,IL-4,IL-6 and IL-8 levels in patients with bronchial asthma j udgment can be used as an important predictor,and it is worthy of clinical application.