HPLC法测定枳实黄酮有效部位中3种二氢黄酮的含量

目的:建立以高效液相色谱法同时测定枳实黄酮有效部位中柚皮苷、橙皮苷和新橙皮苷含量的方法。方法:色谱柱为Hy-persil C18(250mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸水溶液(20:80),流速为1.0mL.min-1,柱温为30℃,检测波长为283nm。结果:柚皮苷、橙皮苷和新橙皮苷的进样量分别在0.44～2.20μg(r=0.9995)、0.0256～0.1280μg(r=0.9993)、0.54～2.70μg(r=0.9999)范围内与各自峰面积积分值呈良好的线性关系;三者平均回收率分别为99.41%、100.33%、99.69%,RSD分别为1.14%、1.47%、1.16%。结论:本方法简便、准确、可靠,可用于枳实的质量控制。

Determination of Three Flavanones in the Effective Fraction of Flavone in Fructus Aurantii Immaturus by HPLC

OBJECTIVE： To establish an HPLC method for simultaneous determination of Naringin, Hesperidin and Neohesperidin in the effective fraction of flavone in Fructus Aurantii Immaturus. METHODS： Hypersil C18 column（250 mm × 4.6 mm, 5μm） was used with the mobile phase consisted of acetonitrile -0.1% phosphoric acid solution （20 ： 80） at a flow rate of 1.0 mL · min^- 1, and the detection wavelength was set at 283 nm and the column temperature was maintained at 30 ℃. RESULTS： The calibration curves of Naringin, Hesperidin and Neohesperidin were in good linearity over the ranges of 0.44- 2.20μg（ r = 0. 999 5）, 0. 025 6- 0.128 0 μg （ r = 0. 999 3）, 0.54 - 2.70μg（ r = 0. 999 9）, respectively.And the average recoveries for the three constituents were 99.41%, 100.33% and 99.69%, respectively with RSD at 1.14%, 1.47%, and 1.16%, respectively. CONCLUSION： The method is simple, accurate and reliable, and can be used for the quality control of Fructus Aurantii Immaturus.