| 低剂量X射线全身照射对小鼠T淋巴细胞钙激活钾通道的影响 |
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| 引用本文： | 孙成文,安刚,钟国赣,战术,江岩,刘树铮,张迎春.低剂量X射线全身照射对小鼠T淋巴细胞钙激活钾通道的影响[J].中华放射医学与防护杂志,1996,16(3):154-156. |
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| 作者姓名： | 孙成文 安刚 钟国赣 战术 江岩 刘树铮 张迎春 |
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| 作者单位： | 白求恩医科大学生理教研室，卫生部放射生物重点实验室 |
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| 摘 要： | 本文作者应用连细胞膜片钳技术，观察了低剂量Ｘ射线全身照射对小鼠Ｔ淋巴细胞钙激活钾通道［Ｋ（Ｃａ）］的影响。发现低剂量照射使ＣｏｎＡ（２０μｇ／ｍｌ）引起的Ｋ（Ｃａ）通道开放增强，表现为开放时间延长、开放概率增加，而对单通道电流幅值无明显影响。在淋巴细胞浸浴液中加入ＣｏｎＡ后３分钟，照射组动物淋巴细胞Ｋ（Ｃａ）通道开放概率明显高于对照组。而且照射后４～２４小时，Ｋ（Ｃａ）通道的激活随时间推移而逐步增强。所以Ｋ（Ｃａ）通道在低剂量放射增强免疫的机制中可能具有重要的意义。
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| 关 键 词： | 膜片钳，钙依赖性钾通道，低剂量辐射，免疫增强，T淋巴细胞 |
| 收稿时间： | 1995/3/21 0:00:00 |
| 修稿时间： | 1995/7/14 0:00:00 |
Effect of low dose X-rays on Ca2+-activated K+ channels in mouse thymic lymphocytes |
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| Sun Chengwen,An Gang,Zhong Guogan.Effect of low dose X-rays on Ca2+-activated K+ channels in mouse thymic lymphocytes[J].Chinese Journal of Radiological Medicine and Protection,1996,16(3):154-156. |
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| Authors: | Sun Chengwen An Gang Zhong Guogan |
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| Abstract: | The effect of low dose X-rays on Ca2 -activated K channelsK(ca)] in mouse thymic lymphocytes was observed using the cell-attached configuration of patch clamp technique.It is shown that low dose X-rays can elevate the open times and openstate probabilities of K(ca) channels,without apparent influence on the current flowing through the channels.After 3 min exposure of lymphocytes to Con A the open-state probabilitis of K(ca) channels in irradiated group were higher than those in control group.It was observed that from 4 to 24h after irradiation the activity of K(ca) chanels increased with time. K(ca)channels are important in the molecular mechanism of immutnoenhancement following low dose irradiation. |
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| Keywords: | Patch clamp Ca2+-activated K+ channel low dose radiation thymic lymphocytes immunoenhancement |
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