| 白纹伊蚊3日龄成蚊饱血前后唾液腺总蛋白谱 |
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| 引用本文： | 吴家红,孙宇,程金芝,陈璐.白纹伊蚊3日龄成蚊饱血前后唾液腺总蛋白谱[J].中国媒介生物学及控制杂志,2009,20(2):102-104. |
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| 作者姓名： | 吴家红 孙宇 程金芝 陈璐 |
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| 作者单位： | 贵阳医学院寄生虫学教研室/现代病原生物学基础实验室,贵阳,550004 |
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| 基金项目： | 国家自然科学基金，贵州省省长基金 |
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| 摘 要： | 目的探讨3日龄白纹伊蚊雌蚊吸食糖水组和饱血组唾液腺总蛋白谱的差异。方法分别采用Pierce蛋白定量试剂盒和SDS-PAGE电泳分析白纹伊蚊3日龄吸食糖水组和饱血组成蚊唾液腺蛋白质浓度和蛋白谱。结果糖水组蚊虫单对唾液腺蛋白质质量约为（1．243Э0．460）μg，饱血组约为（0．945±0．460）μg；SDS—PAGE检测到糖水组唾液腺总蛋白有12条主带，糖水组与饱血组唾液腺总蛋白条带没有变化。结论白纹伊蚊雌蚊饱血后唾液腺总蛋白无特异条带诱导，但饱血后单对唾液腺总蛋白下降。
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| 关 键 词： | 白纹伊蚊 唾液腺 SDS.PAGE |
Total protein spectrum of salivary gland from Aedes albopictus aged 3 days after a blood meal |
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| WU Jia-hong,SUN Yu,CHENG Jin-zhi,CHEN Lu.Total protein spectrum of salivary gland from Aedes albopictus aged 3 days after a blood meal[J].Chinese Journal of Vector Biology and Control,2009,20(2):102-104. |
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| Authors: | WU Jia-hong SUN Yu CHENG Jin-zhi CHEN Lu |
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| Institution: | . (Department of Parasitology/Basic Laboratory of Modern Pathogenic Biology, Guiyang Medical College, Guiyang, Guizhou 55000d, China) |
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| Abstract: | Objective To detect the difference of the salivary protein spectrum from Aedes albopictus aged 3 days with blood-feed and sugar-feed, respectively. Methods The salivary protein was detected with Pierce BCA Protein Assay Kit and SDS-PAGE. Results The amount of salivary gland proteins in sugar- feed and blood-feed mosquitoes aged 3 days was approximately （1.243 ± 0.460）μg/female and （0.945± 0.460）μg/female, respectively. There were at least twelve major proteins in sugar-feed mosquito salivary glands analyzed by SDS-PAGE, and no difference was detected in the electrophoretic bands between sugar-feed and blood-feed mosquitoes salivary gland. Conclusion No specific protein was induced after Ae.albopictus feeding blood, but the total protein of salivary gland per female decreased after a blood meal. |
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| Keywords: | SDS-PAGE |
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