| 应用高速逆流色谱分离连翘中连翘苷 |
| |
| 引用本文： | 张继全,阮克锋,杜若飞.应用高速逆流色谱分离连翘中连翘苷[J].世界科学技术-中医药现代化,2011,13(5):796-799. |
| |
| 作者姓名： | 张继全 阮克锋 杜若飞 |
| |
| 作者单位： | 上海中医药大学中药现代制剂技术教育部工程研究中心;上海中医药大学中药现代制剂技术教育部工程研究中心;上海张江中药现代制剂技术工程研究中心 |
| |
| 基金项目： | 上海市教委重点学科项目(J50302):中药药剂学,负责人:冯怡;科学技术部国家重大专项项目(2009ZX09502-009):中药制剂物理改性技术研究,负责人:冯怡 |
| |
| 摘 要： | 目的:应用高速逆流色谱技术从连翘药材提取物中分离得到连翘苷。方法:采用两相溶剂系统,四氯化碳-氯仿-甲醇-水的四元体系,上相为固定相,下相为流动相;仪器转速为900rpm,流速为1.2mL.min-1。结果:经过ESI-MS和1H-NMR的鉴定,确认为连翘苷单体化合物,外标法测定纯度为98.8%。结论:本方法可以用于分离制备连翘苷单体。
|
| 关 键 词： | 高速逆流色谱 连翘 连翘苷 |
| 收稿时间： | 2010/10/18 0:00:00 |
| 修稿时间： | 2011/2/12 0:00:00 |
Separation of Phillyrin from Fructus Forsythiae by High-Speed Counter-Current Chromatography |
| |
| Zhang Jiquan,Ruan Kefeng and Du Ruofei.Separation of Phillyrin from Fructus Forsythiae by High-Speed Counter-Current Chromatography[J].World Science and Technology-Modernization of Traditional Chinese Medicine,2011,13(5):796-799. |
| |
| Authors: | Zhang Jiquan Ruan Kefeng and Du Ruofei |
| |
| Institution: | Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Shanghai Zhangjiang Engineering Research Center of Modern Preparation Technology of TCM, Shanghai 201203,China |
| |
| Abstract: | Phillyrin,an active component in the Fructus Forsythiae,was successfully separated from Chinese herbal medicine Fructus Forsythiae by High-Speed Counter-Current Chromatography(HSCCC).The solvent system used for separation was consisted of carbon tetrachloride-chloroform-methanol-water.The upper phase was used as stationary phase and the lower phase was used as mobile phase.The apparatus was rotated at 900 rpm and the mobile phase of solvent was pumped through the column at a flow rate of 1.2 mL/min.The identification by ESI-MS and 1H-NMR proved that the component was phillyrin,and the purity was 98.8%.It is concluded that HSCCC can be used in the separation of phillyrin. |
| |
| Keywords: | |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《世界科学技术-中医药现代化》浏览原始摘要信息 |
| 点击此处可从《世界科学技术-中医药现代化》下载免费的PDF全文 |
