| 紫外诱变选育北虫草新菌种的研究 |
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| 引用本文： | 张 鑫,唐 亮,徐三妹. 紫外诱变选育北虫草新菌种的研究[J]. 世界科学技术-中医药现代化, 2011, 13(3): 567-570 |
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| 作者姓名： | 张 鑫 唐 亮 徐三妹 |
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| 作者单位： | 上海国宝企业发展中心；上海国宝生物工程研究所 上海 201203 |
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| 基金项目： | 科学技术部“十一五”国家863计划重点项目（2007AA021502）：北虫草人工替代品规模化生产关键技术，负责人：唐亮。 |
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| 摘 要： | 针对野生菌种在人工培养基上容易丢失产生子实体的能力,稳定性差,且野生型菌种虫草菌素等生理活性物质含量较低等影响北虫草规模化人工栽培的问题,本文探索利用紫外线对出发菌株P进行诱变处理,以生物转化率、虫草素、腺苷含量为指标,筛选出高产菌株89,其有性子实体中虫草素含量和腺苷含量分别是出发菌株的2.5倍和3.6倍.进一步对其...
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| 关 键 词： | 北虫草 紫外诱变 选育 虫草素 腺苷 遗传稳定性 |
| 收稿时间： | 2011-03-07 |
| 修稿时间： | 2011-04-21 |
Investigation on Breeding of Cordyceps militaris (L.) Strain by Ultraviolet Mutagenesis |
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| Zhang Xin,Tang Liang and Xu Sanmei. Investigation on Breeding of Cordyceps militaris (L.) Strain by Ultraviolet Mutagenesis[J]. World Science and Technology—Modernization of Traditional Chinese Medicine and Materia Medica, 2011, 13(3): 567-570 |
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| Authors: | Zhang Xin Tang Liang Xu Sanmei |
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| Affiliation: | Shanghai Guobao Enterprise Development Center, Shanghai 201203, China; Shanghai Guobao Bioengineering Institute, Shanghai 201203, China |
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| Abstract: | Based on problems existed in the cultivation of Cordyceps militaris (L.) strain, ultraviolet ray was used to mutate original thallus P. Biological conversion rate, content of cordycepin and adenosine were evaluated as indexes. The amount of screened high output strain was 89. The content of cordycepin and adenosine were 2.5 times and 3.6 times than before, respectively. Genetic stability experiments indicated the strains were stable and can be used as parent thallus in large-scale. |
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| Keywords: | Cordyceps militaris ultraviolet mutagenesis breeding cordycepin adenosine genetic stability |
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