| 基于ITS2 序列的女贞子原植物及其混伪品的分子鉴定 |
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| 引用本文： | 李美妮,韩蕊莲,韩建萍,刘美子,姚辉,宋经元. 基于ITS2 序列的女贞子原植物及其混伪品的分子鉴定[J]. 世界科学技术-中医药现代化, 2011, 13(4): 644-649 |
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| 作者姓名： | 李美妮 韩蕊莲 韩建萍 刘美子 姚辉 宋经元 |
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| 作者单位： | 西北农林科技大学生命科学学院 杨凌 712100;中国医学科学院北京协和医学院药用植物研究所,中草药物质基础与资源利用教育部重点实验室 北京100193;西北农林科技大学生命科学学院 杨凌 712100;中国医学科学院北京协和医学院药用植物研究所;中草药物质基础与资源利用教育部重点实验室 北京 100193;中国医学科学院北京协和医学院药用植物研究所;中草药物质基础与资源利用教育部重点实验室 北京 100193;中国医学科学院北京协和医学院药用植物研究所;中草药物质基础与资源利用教育部重点实验室 北京 100195;中国医学科学院北京协和医学院药用植物研究所;中草药物质基础与资源利用教育部重点实验室 北京 100197 |
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| 基金项目： | 国家自然科学基金青年基金项目（81001608）：基于全叶绿体基因组分析筛选乌头属药用植物DNA条形码序列，负责人：韩建萍 |
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| 摘 要： | 目的:通过分析女贞子原植物及其混用品和伪品的ITS2序列,探究鉴定女贞及其混伪品的新方法。方法:采用CodonCode Aligner进行序列拼接,MEGA4.1计算女贞及其混伪品的种内、种间的K2P距离,并基于K2P模型构建NJ树,最后应用Schultz等建立的ITS2数据库和网站预测二级结构。结果:女贞的最小种间K2P距离(3.2%)大于种内最大K2P距离(0.90%),NJ树中女贞的不同来源样品聚为一支。女贞与其混伪品的二级结构的分子形态均有差异。结论I:TS2序列可以作为鉴定女贞子原植物及其混伪品的条形码序列,并且该序列在中药材原植物的鉴定中具有很大的潜力。
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| 关 键 词： | 女贞 混伪品 分子鉴定 ITS2序列 |
| 收稿时间： | 2011-06-29 |
| 修稿时间： | 2011-08-05 |
Discrimination of the Original Plant of Ligustri Lucidi Fructus and Its Adulterants Using ITS2 Sequence |
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| Li Meini,Han Ruilian,Han Jianping,Liu Meizi,Yao Hui and Song Jingyuan. Discrimination of the Original Plant of Ligustri Lucidi Fructus and Its Adulterants Using ITS2 Sequence[J]. World Science and Technology—Modernization of Traditional Chinese Medicine and Materia Medica, 2011, 13(4): 644-649 |
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| Authors: | Li Meini Han Ruilian Han Jianping Liu Meizi Yao Hui Song Jingyuan |
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| Affiliation: | Colledge of Life Science, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China; The Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine,Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy ofMedical Sciences & Peking Union Medical College, Beijing 100193, China;Colledge of Life Science, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China |
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| Abstract: | Objective: To explore a new method to identify the original plant of Ligustri Lucidi Fructus from its adulterants by the ITS2 regions. Methods: Sequences were assembled using CodonCode Aligner. K2P distances were calculated and NJ Tree was performed by MEGA 4.1. Secondary structures were predicted by the ITS2 database and websites. Results: The minimum interspecific K2P distance (3.2%) was obviously higher than the maximum intraspecific one (0.90%). The different samples of Ligustrum lucidum were clustered into one clade. The molecular morphology of secondary structures of ITS2 was compared between Ligustrum lucidum and its adulterants. Conclusions:The ITS2 region could be used to discriminate the original plant of Ligustri Lucidi Fructus and its adulterants. |
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| Keywords: | Ligustrum lucidum adulterants molecular identification ITS2 sequence |
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