我国金银花栽培品种的遗传多样性

目的:对国内金银花主产区的栽培品种进行遗传多样性分析,为了解不同产区金银花栽培品种的亲缘关系以及金银花新品种选育提供参考。方法:利用RAPD技术分析国内不同产区采集的11份栽培金银花和1份山银花样品,用NTSYS 2.10e软件对统计结果进行聚类分析。结果:17条RAPD引物共扩增出150条清晰可辨的DNA条带,其中113条在不同样品间具有多态性,通过聚类分析可将12份金银花样品分为4个大类。结论:河南封丘、河北巨鹿的金银花品种遗传差异较小,品种较为单一;山东平邑的金银花种质资源相对丰富。研究还发现,不同金银花种源间的遗传关系与地理分布存在相关性。

Genetic Diversity of Lonicera Japonica Cultivars in China

This study aimed to analyze the genetic diversity of Lonicera japonica cultivars, and provide preference on understanding the genetic relationship of Lonicera japonica cultivars in main producing areas of China and developing new varieties of Lonicera japonica. In the study, a total of eleven Lonicera japonica cultivars and one Lonicera japonica variety were analyzed by RAPD. And statistic data were clustered by software NTSYS version 2.10e. The results showed that 150 distinctive DNA bands were amplified by 17 RAPD primers. And 113 bands ofthem have polymorphism among Lonicera japonica samples. Furthermore, twelve Lonicera japonica samples can bedivided into four groups by clustering analysis. It is concluded that genetic diversity of Lonicera japonica cultivars from Pingyi of Shandong province was comparatively higher than those from Fengqiu of Henan province and Julu of Hebei province. And genetic relationships of Lonicera japonica samples from different producing areas were corre lated with their geographic distribution.