| 活体柞蚕蛹复壮北虫草菌种初探 |
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| 引用本文： | 张 鑫,唐 亮,徐三妹.活体柞蚕蛹复壮北虫草菌种初探[J].世界科学技术-中医药现代化,2011,13(3):571-574. |
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| 作者姓名： | 张 鑫 唐 亮 徐三妹 |
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| 作者单位： | 上海国宝企业发展中心；上海国宝生物工程研究所 上海 201203 |
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| 基金项目： | 科学技术部“十一五”国家863计划重点项目（2007AA021502）：北冬虫夏草人工替代品规模化生产关键技术，负责人：唐亮。 |
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| 摘 要： | 目的：对北虫草菌株进行复壮处理以提高菌种活力。方法：用一株人工培育的北虫草菌株P感染刚化蛹的活体柞蚕蛹，蛹体近头部长出子实体后，待子实体近成熟期时采集孢子进行有性繁殖对原菌株进行复壮。结果：复壮后菌株10-2的子实体产量和生物转化率分别是原来菌株P的1.2倍和1.5倍。结论：在表面消毒过的活体柞蚕蛹上接种液体菌株活化复壮菌种的方法是可行的。
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| 关 键 词： | 活体 柞蚕蛹 感染 复壮 |
| 收稿时间： | 3/7/2011 12:00:00 AM |
| 修稿时间： | 2011/4/21 0:00:00 |
Rejuvenation Method on Cordyceps militaris Using Antheraea pernyi Guerin-Meneville Living Body |
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| Zhang Xin,Tang Liang and Xu Sanmei.Rejuvenation Method on Cordyceps militaris Using Antheraea pernyi Guerin-Meneville Living Body[J].World Science and Technology-Modernization of Traditional Chinese Medicine,2011,13(3):571-574. |
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| Authors: | Zhang Xin Tang Liang and Xu Sanmei |
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| Institution: | Shanghai Guobao Enterprise Development Center, Shanghai 201203, China; Shanghai Guobao Bioengineering Institute, Shanghai 201203, China |
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| Abstract: | This study aimed to increase strain output by using rejuvenation method on Cordyceps militaris strain. Just after pupation, the Antheraea pernyi Guerin-Meneville living body was infected by a manual cultivation for Cordyceps militaris (L.) strain P. After pupal body grew a new sporocarp near the head, the spores were gathered and used for the sexual reproduction on the rejuvenation of strain P at maturity of sporophores. The tracking result of solid medium indicated output and the biological conversion rate of strain were 1.2 times and 1.5 times than strain P, respectively. The conclusion is that using rejuvenation method on Cordyceps militaris strain with Antheraea pernyi Guerin-Meneville living body is practical. |
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| Keywords: | Living body Antheraea pernyi Guerin-Meneville infection rejuvenation |
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