槲皮素固体脂质纳米粒的包封率与载药量测定

目的:建立槲皮素固体脂质纳米粒(SLN)的包封率和载药量测定方法。方法:采用高速离心-高效液相色谱法。色谱柱为DiamonsiL-C18(250mm×4.6mm,5μm),流动相为甲醇-4.3%乙酸溶液(55∶45),流速为1.0mL·min-1,检测波长为254nm,柱温为30℃。结果:槲皮素检测浓度在2.0～200.0μg·mL-1范围内与峰面积积分值呈良好线性关系(r=0.9996);平均回收率为97.83%,RSD=1.03%(n=6)。该条件下,槲皮素SLN的包封率为80.2%,载药量为1.7%。结论:所建方法便捷、可靠,可用于SLN包封率与载药量的测定。

Determination of Drug-loading Rate and Encapsulation Efficiency of Quercetin Solid Lipid Nanoparticles

OBJECTIVE：To establish the determination method of the drug-loading rate and encapsulation efficiency of Quercetin solid lipid nanoparticles（QUE-SLNs）.METHODS：A high-velocity centrifuge-HPLC was adopted.The determination was performed on DiamonsiL-C18（250 mm×4.6 mm,5 μm）column with mobile phase consisted of methanol-4.3% acetic acid solution（55 ：45） at flow rate of 1.0 mL·min-1.The detection wavelength was set at 254 nm and column temperature was 30 ℃.RESULTS：The linear range of quercetin was 2.0～200.0 μg·mL-1（r=0.999 6） with an average recovery of 97.83%（RSD=1.03%,n=6）.The encapsulation efficiency of QUE-SLNs was 80.2% and the drug-loading rate was 1.7%.CONCLUSION：The method is simple,convenient and reliable.It can be used for the determination of drug-loading rate and encapsulation efficiency of SLN.