| RP-HPLC法测定变通丸中盐酸小檗碱的含量 |
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| 引用本文： | 李明月,杨惊宇,刘西京,倪建新,蔡昌龙.RP-HPLC法测定变通丸中盐酸小檗碱的含量[J].中国药房,2011(27):2555-2556. |
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| 作者姓名： | 李明月 杨惊宇 刘西京 倪建新 蔡昌龙 |
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| 作者单位： | 广东东莞市塘厦医院;广东深圳市宝安区中医院;广东深圳市宝安区人民医院; |
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| 基金项目： | 广东省中医药局科研课题(2010310) |
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| 摘 要： | 目的:建立测定变通丸中盐酸小檗碱含量的方法。方法:采用反相高效液相色谱法。色谱柱为SunFireTMC18(250mm×4.6mmi.d.,5μm),流动相为1.5mmo·lL-1十二烷基硫酸钠溶液(以磷酸调pH5.0)-乙腈(梯度洗脱),流速为0.8mL·min-1,柱温为25℃,检测波长为265nm。结果:盐酸小檗碱的质量浓度在60.8～304.0μg·mL-1范围内与峰面积积分值呈良好线性关系;平均加样回收率为97.59%,RSD=1.34%(n=6)。结论:本法简便、快速、重复性好,结果准确、可靠,可作为变通丸中盐酸小檗碱含量测定的方法。
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| 关 键 词： | 反相高效液相色谱法 变通丸 盐酸小檗碱 含量测定 |
Content Determination of Berberine Hydrochloride in Biantong Pills by RP-HPLC |
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| LI Ming-yue,CAI Chang-long YANG Jing-yu LIU Xi-jing,NI Jian-xin.Content Determination of Berberine Hydrochloride in Biantong Pills by RP-HPLC[J].China Pharmacy,2011(27):2555-2556. |
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| Authors: | LI Ming-yue CAI Chang-long YANG Jing-yu LIU Xi-jing NI Jian-xin |
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| Institution: | LI Ming-yue,CAI Chang-long(Dongguang Tangxia Hospital of Guangdong Province,Dongguang 523721,China) YANG Jing-yu(Shengzhen Baoan District Hospital of Traditional Chinese Medicine of Guangdong Province,Shengzhen 518133,China) LIU Xi-jing,NI Jian-xin(Shengzhen Baoan District People's Hospital of Guangdong Province,China) |
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| Abstract: | OBJECTIVE：To establish the method for the content determination of berberine hydrochloride in Biantong pills.METHODS：RP-HPLC method was adopted.The determination was performed on SunFireTM C18 column（250 mm×4.6 mm,5 μm） with mobile phase consisted of 1.5 mmol·L-1 sodium dodecyl sulfate（pH value adjusted to 5.0 with phosphoric acid）-acetonitrile（gradient elution） at flow rate of 0.8 mL·min-1.The column temperature was 25 ℃ and detection wavelength was set at 265 nm.RESULTS：The linear range of berberine hydrochloride was 60.8～304.0 μg·mL-1 with an average recovery of 97.59%（RSD=1.34%,n=6）.CONCLUSION：This method is simple,rapid,reproducible,accurate and reliable for the content determination of berberine hydrochloride in Biantong pills. |
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| Keywords: | RP-HPLC Biantong pills Berberine hydrochloride Content determination |
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