芒果苷在鸭体内抑制鸭乙型肝炎病毒感染的实验研究

目的]观察芒果苷对鸭乙型肝炎病毒感染模型的抑制作用。方法]采用1日龄北京鸭静脉注射强阳性鸭乙型肝炎病毒后,口服给予芒果苷50、100和200mg/kg 3个剂量,每日2次,连续给药10d,于给药前、给药后第5、10天以及停药后第3天采血,分离血清,采用斑点杂交方法及放射自显影方法,观察鸭血清乙肝病毒DNA(DHBV-DNA)的动态变化。结果]芒果苷100mg/kg、200mg/kg在给药期间对DHBV-DNA有明显抑制作用;且在停药后未见明显反跳现象。结论]提示芒果苷有良好的抑制DHBV感染作用,且停药后不易出现反跳现象。

INHIBITORY EFFECT OF MANGIFERIN ON DUCK HEPATITIS B VIRUS (DHBV) DNA IN VIVO

Objective]To investigate the inhibitory effect of Mangiferin on duck hepatitis B virus DNA in vivo.Methods]1-day-old Peking ducks were administered orally with Mangiferin,either of 50 mg/kg,or 100 mg/kg,or 200mg/kg for twice a day,10 days on end after intravenous administration of DHBV.Blood collection was performed before the treatment,which was also applied to the 5th,10th day and 3 days after the treatment.Dynamic changes of DHBV DNA were observed by dot-blot hybridization test on the isolated serum.Results]Mangiferin markedly inhabited the DHBV DNA when the dose of which was 100mg/kg or 200mg/kg.There was no obvious rebound phenomenon shown after the treatment.Conclusion]Mangiferin has favorable inhabiting effect on DHBV DNA.Rebound phenomenon rarely occurs after administration of Mangiferin.