微量稀释法对肿瘤患者术后合并感染ESBLs菌株的检测验证

目的：用新型微量稀释法对肿瘤患者术后合并感染超广谱β－内酰胺酶菌株进行检测验证。方法：双纸片法初试微量稀释法验证，对２７株肺炎克雷伯氏菌和３２株大肠埃希氏前进行赵广谱β－内酰胺酶（Ｅｘｔｅｎｄｅｄ－Ｓｐｅｆｃ－ｔｒｕｍ－ｔｕｃｔａｍａｓｅｓ，简称ＥＳＢＬｓ）检测。结果：显示肺炎克雷伯氏菌和大肠埃希氏菌ＥＳＢＬｓ携带率分别为１４．８％（４／２７）和９．４％（３／３２）。结论：微量稀释法是一种新型的验证方法，不但可以测ＥＳＢＬｓ，还可以同时测多种头孢三代类抗生素的ＭＩＣ，具有不易漏检，重复性好，准确，操作简便，终点易于判断的优点。

A New Microdilution Test for Detecting Postoperative Infection ofExtended Spectrum Lactamases in Cancer Patients

Aim: To study the identification of ESBLs (extended spectrum lactamases) in postopera-tive infection in cancer patients. Methods: The double - disc test (a new microdilution test) was used toidentify the primary state of ESBLs- Results: The positive rates of ESBLs in klebsiella Pneumoniae(K. P) and Escherichia coli (E. C) were 14. 8% (4/27) and 9-4% (3/32) respectively. Conclu.sion: The microdilution test is a new test which not only can detect ESBLs but also is able to test theMICs of several third generation of Cephalosporins. It is simple, accurate with good repeatability andeasy judgement of the end point.