荧光探针PCR与膜芯片技术在女性生殖道HPV分型的对比研究

目的探讨荧光探针PCR与膜芯片分型技术在检测女性生殖道人乳头状瘤病毒中的应用情况，并进行临床应用评价。方法门诊230例妇女同时利用荧光探针PCR与膜芯片分型技术检测其宫颈脱落细胞HPV-DNA，其中70例进行阴道镜宫颈组织病理活检。荧光探针PCR定性检测14种高危型HPV病毒(包括HPV16，18，31，33，35，39，45，51，52，56，58，59，68和66)，同时对其中的HPV16和HPV18型进行分型检测。膜芯片分型技术检测23种HPV型，包括上述14种HPV，另外还有HPV53，73，82和83高危型和5种低危型HPV病毒(HPV6，11，42，43和81)。结果荧光探针PCR定性检测HPV的阳性率为11.30%(26/230)，膜芯片分型技术检测HPV的总阳性率为19.57%(45/230)，高于荧光探针PCR定性(P<0.05)。二者检测14种高危型HPV病毒总的符合率为94.35%。在70例病理活检中荧光探针PCR定性和膜芯片分型技术检测为HPV-DNA阳性的妇女中，宫颈上皮内瘤变2级以上，分别为26.92%(7/26)及22.22%(10/45)，分别高于其HPV-DNA阴性对照组的4.55% (2/44)及4.00% (1/25)(P<0.05)。结论荧光探针PCR与膜芯片分型技术比较，在检测常见14种高危型HPV方面结果具有较好的一致性，更适合大规模健康体检中的应用。然而两种方法又有其不同的优势，应结合具体情况进行应用。

Study on Comparing with the FluorescenceProbe PCR and Film Chip Technology for Genotyping HumanPapilloma Virus in the Female Reproductive Tract

ObjectiveTo evaluate the clinical application of the fluorescence probe PCR and film chip genotyping technology for detecting human papilloma virus in the female reproductive tract．MethodsA total of 230 outpatient women were checked for 14 high-risk HPV genotypes (HPV16，18，31，33，35，39，45，51，52，56，58，59，68 and 66)．At the same time，typing detection of the HPV16 and HPV18 were taken by the fluorescence probe PCR，and were detected to 23 HPV genotypes (HPV16，18，31，33，35，39，45，51，52，56，58，59，68，66，53，73，82，83 and 5 low-risk types of HPV virus) by film chip genotyping technology．ResultsThe positive rate of the fluorescence probe PCR was 11.30% (26/230)，and the total positive rate of film chip genotyping technology was 19.57% (45/230)，and film chip genotyping technology had more positive rate than the fluorescence probe PCR in detecting HPV (P<0.05)．The total coincidence rate of two methods was 94.35%．In 70 patients with pathological biopsy，the rate above cervical intraepithelial neoplasia Ⅱ was 26.92% (7/26) and 22.22% (10/45) in the fluorescence probe PCR and film chip genotyping technology test positive samples， which was higher than 4.55% (2/44) and 4.00% (1/25) in the fluorescence probe PCR and film chip genotyping technology test negative samples respectively (P<0.05)．ConclusionIt had good consistency for detection of the 14 high-risk HPV genotypes by the fluorescence probe PCR and film chip genotyping technology，and had the important significance for HPV infection，early cervical cancer discovery，prevention and treatment．