荷载Zebularine的聚合物胶束复合体纳米颗粒对兔眼晶状体后囊膜混浊的预防作用

目的　研究荷载Ｚｅｂｕｌａｒｉｎｅ（Ｚｅｂ）的聚合物胶束复合体（ＭｅＰＥＧ-ＰＣＬ）纳米颗粒（ｎａｎｏｐａｒｔｉｃｌｅｓ，ＮＰｓ）对兔晶状体后囊膜混浊（ｐｏｓｔｅｒｉｏｒｃａｐｓｕｌｅｏｐａｃｉｆｉｃａｔｉｏｎ，ＰＣＯ）的预防作用。方法　选取３２只新西兰白兔随机分为生理盐水对照组（Ｃ组）、游离Ｚｅｂ组（ＺｅｂＦ组）、荷载Ｚｅｂ的ＭｅＰＥＧ-ＰＣＬＮＰｓ组（ＺｅｂＮＰｓ组）、ＭｅＰＥＧ-ＰＣＬ空载颗粒组（ＮＰｓ组），所有动物行晶状体摘出＋人工晶状体植入术，Ｃ组术毕球结膜下注射生理盐水０．１ｍＬ，ＺｅｂＦ组注射０．４ｇ·Ｌ－１的游离Ｚｅｂ０．１ｍＬ，ＺｅｂＮＰｓ组注射０．４ｇ·Ｌ－１荷载Ｚｅｂ的ＭｅＰＥＧ-ＰＣＬＮＰｓ０．１ｍＬ，ＮＰｓ组注射ＭｅＰＥＧ-ＰＣＬ空载颗粒０．１ｍＬ。术后采用裂隙灯观察前房反应和ＰＣＯ分级，动态测量眼压，高效液相色谱法测定房水中Ｚｅｂ浓度。结果　术后１ｄ、３ｄ各组间前房反应差异无统计学意义（Ｈ＝５．２１，Ｐ＝０．５９）。术后各组间对应时间点眼压差异均无统计学意义（均为Ｐ＞０．０５）。术后１ｄ、２ｄＺｅｂＮＰｓ组房水中Ｚｅｂ含量显著高于ＺｅｂＦ组（均为Ｐ＜０．０１），术后３ｄＺｅｂＦ组房水中已经检测不到Ｚｅｂ，术后２ｄＺｅｂＮＰｓ组房水中药物含量最高，随后降低。术后１０周ＺｅｂＦ组、ＮＰｓ组、Ｃ组均发生了明显的ＰＣＯ，ＺｅｂＮＰｓ组ＰＣＯ较ＺｅｂＦ组显著减轻（Ｈ＝９．９１，Ｐ＜０．０５）。结论　荷载Ｚｅｂ的ＭｅＰＥＧ-ＰＣＬＮＰｓ可通过增高房水中Ｚｅｂ浓度有效减轻兔眼晶状体摘出术后ＰＣＯ程度。

Effects of Zebularine loaded MePEG-PCL nanoparticles on posterior capsule opacification

Objective To investigate the preventive effects of zebularine ( Zeb) loaded Poly ( ethylene glycol) -block-poly ( a-caprolactone ) methyl ether ( MePEG-PCL ) nanoparticles( NPs) on the posterior capsule opacification( PCO) of rabbit. Methods Thirty-two Zealand white rabbits were divided into control group ( group C) , ZebF group , ZebNPs group , NPs group , randomly. All animals were treated with cataract extraction plus intraocular lens implantation. Immediately after completion of surgery , 0. I mL normal saline.0. I mL saline contairung 0. 4 g . L-l Zeb .0. I mL zeb -loaded MePEGPCL NPs containing 0.4 g . L-l Zeb and 0. I mL MePEG-PCL empty NPs were subconjunctivally injected in group C , ZebF group , ZebNPs group , NPs group , respectively. The degrees of anterior chamber response and severity degree of PCO were observed by slit lamp micrographscopy , and intraocular pressure ( IOP) was measured continuously , Zeb concentration in the aqueous humor was assayed by HPLC. Results There was no difference among all groups in degree of anterior chamber response at the I day and 3 days after operation ( H = 5. 21 ,P = 0. 59 ) . as well in IOP at the same time points of the I day , 2 days ,3 days and 7 days after operation ( all P > 0. 05 ) . The concentrations of Zeb in the aqueous humor at the I day and 2 days after operation in ZebNPs group was significantly higher than those in ZebF group ( all P < 0. 01 ) . Zeb did not be detected at 3 days after operation in ZebF group , and the concentrations of Zeb in the aqueous humor in ZebNPs group peaked at 3 days after operation , then decreased. The severity of PCO in ZebNPs group was decreased compared with ZebF group ( H = 9. 91 .P < 0. 05 ) . Conclusion MePEG-PCL zeb-loaded NPs can lessen the severity of PCO after cataract extraction by increasing the concentration of Zeb in the aqueous humor.