Wnt5a在慢性根尖周炎病损中的表达及临床意义

目的检测慢性根尖周炎根尖病损区Wnt5a的水平与细胞定位,探讨Wnt5a与炎症细胞浸润程度的关系,以及Wnt5a在慢性根尖周炎发展中的作用及意义。方法选取10例慢性根尖周炎牙作为实验组,根据根尖区组织的H-E染色,依炎症细胞浸润程度将病例组分为轻中度组、重度组。5例因正畸需要而拔除的健康牙作为对照组。采用实时定量PCR(real-time PCR,RT-PCR)从mRNA水平检测样本Wnt5a的表达;免疫组织化学法检测样本中Wnt5a蛋白的表达,应用图像分析软件检测Wnt5a蛋白表达水平,分析Wnt5a表达高低与炎症程度的关系。采用SPSS13.0软件包对数据进行统计学分析。结果Wnt5a在实验组与对照组均有表达,实验组中Wnt5a mRNA表达水平显著高于对照组(P<0.05)。免疫组织化学结果显示,重度炎症组Wnt5a的表达水平显著高于对照组(P<0.01);轻中度组Wnt5a的表达水平显著高于对照组(P<0.05);重度炎症组中Wnt5a的表达水平显著高于轻中度炎症组(P<0.05)。结论Wnt5a与慢性根尖周炎的炎症程度相关,可能参与了病变的始动过程,推测Wnt5a在慢性根尖周炎的发生、发展中起着一定作用。

Expression of Wnt5a in chronic apical periodontitis and its clinical significance

PURPOSE: To detect the expression of Wnt5a in lesions of chronic apical periodontitis and determine the relationship between expression of Wnt5a and inflammation degree. METHODS: Ten patients with chronic apical periodontitis and 5 healthy controls were enrolled in this study. According to the inflammatory cell infiltration, the specimens were divided into 2 groups: severe inflammation group and mild inflammation group. The expression of Wnt5a was measured by real-time PCR (RT-PCR) and immunohistochemical analysis in the lesions of chronic apical periodontitis. The amount of Wnt5 expression was assayed and compared in different inflammation levels. Statistical analysis was performed using SPSS 13.0 software package. RESULTS: Wnt5a was detected in both groups. Expression of Wnt5a mRNA in patients were significantly higher than the controls (P<0.05). According to inflammation level, the positive expression rate of Wnt5a in severe inflammation group was significantly higher than the controls (P<0.01), and Wnt5a positive expression in mild inflammation group was also significantly higher than the controls (P<0.05). The expression of Wnt5a was significantly different between severe inflammation group and mild inflammation group (P<0.05). CONCLUSIONS: The expression of Wnt5a increases as the severity of tissue inflammation increases , which indicates that Wnt5a plays an important role in the development of chronic apical periodontitis.