肝素结合性表皮生长因子在翼状胬肉中的表达及意义

目的　研究肝素结合性表皮生长因子（ｈｅｐａｒｉｎ-ｂｉｎｄｉｎｇｅｐｉｄｅｒｍａｌｇｒｏｗｔｈｆａｃｔｏｒ，ＨＢ-ＥＧＦ）在翼状胬肉组织中的表达与分布，探讨其在翼状胬肉发生过程中的作用。方法　收集２０１３年８月至２０１４年８月在南昌大学眼科医院行翼状胬肉切除的４０例标本，采用免疫组织化学染色和Ｗｅｓｔｅｒｎｂｌｏｔ分别检测２２例初发与１８例复发翼状胬肉标本中ＨＢ-ＥＧＦ的表达情况，并与正常对照组结膜标本进行比较。结果　ＨＢ-ＥＧＦ在初发型翼状胬肉组织的头部相对表达值为２．７７、体部相对表达值为１．７６，正常结膜相对表达值为１．３７。复发型胬肉组织中ＨＢ-ＥＧＦ在其头部相对表达值为１．９５、体部相对表达值为１.５７，正常结膜相对表达值为０．９６。ＨＢ-ＥＧＦ在初发型和复发型翼状胬肉组织中均主要定位于上皮细胞和血管内皮细胞的胞浆中，以上皮细胞表达最为显著。相同标本数复发型翼状胬肉头部组织中ＨＢ-ＥＧＦ的相对表达值为４．３０，为正常对照结膜（相对表达值为１．０９）的３．９倍、为初发型（相对表达值为１．３２）翼状胬肉头部组织３．１倍，且初发型翼状胬肉头部组织表达量为正常对照组的１．３倍。结论　高水平的ＨＢ-ＥＧＦ可能是翼状胬肉发生与复发的重要因素，提示可以把ＨＢ-ＥＧＦ作为干预靶点来控制翼状胬肉的发生与复发。

Expression of HB-EGF in human pterygium and its significance

Objective To investigate the expression and distribution of heparin-binding epidermal growth factor ( HB-EGF) in pathogenesis of human pterygium, and study the role of its played. Methods Between August 2013 and August 2014 ,40 cases of pterygium exasion specimens were collected and produced in Affiliated Eye Hospital of Nanchang University. The expression differences of HB-EGF between pterygium and normal conjunctiva tissue from the same pterygium patient were compared with immunohistochemistry and Western blot. Also we used the same technique to examine the expressions and distribution of HG-EGF in pterygium tissue of 22 cases with first operation and 18 recurrent cases as well as the expression in 15 specimens of normal conjunctiva from patients operated for other disorders. Results In primary pterygium group , the relative intensities of HB-EGF in pterygium-head was 2. 77. pterygium-body was l. 76 and normal conjunctiva tissue was l. 37. In recurrent pterygium group ,the relative intensities of HB-EGF in pterygiumhead was l. 95 , pterygium-body was l. 57 and normal conjunctiva tissue was 0. 96. And HB-EGF largely distributed in the endochylema of epithelial cells and vascular endothelial cells of pterygium , especially in epithelial cells. The relative intensities of total HB-EGF in recurrent group was 4. 30 which was 3. 9 times over the normal conjunctive group ( the relative intensities was l. 09 ) .3. I times as strong as the primary group ( the relative intensities was l. 32) . and the primary group was l. 3 times over the normal conjunctive group. Conclusion Over-expression of HB-EGF is an important cause of development and recurrence of pterygium, which indicate that HB-EGF can be set as the therapeutic target for controlling the development and recurrence of pterygium.