IL-35在慢性牙周炎血清及龈沟液中的表达及相关性

目的通过检测牙周炎患者血清及龈沟液中白细胞介素35( IL-35)的水平,分析其与临床指标的相关性,初步探讨IL-35在牙周疾病过程中的变化情况。方法按纳入标准选择20例中、重度牙周炎患者作为实验组,20例健康人群作为对照组;记录患者的一般信息、牙周袋探诊深度( PD)和临床附着丧失( CAL) 情况;收集静脉血清及龈沟液样本,采用酶联免疫吸附法(ELISA)测定血清及龈沟液中的IL-35水平;利用SPSS19.0软件包进行Pearson秩相关检验,分析IL-35的表达及与临床指标的关系。结果实验组血清及龈沟液中IL-35平均浓度分别为（330.42±19.23）ng/mL、(205.56±14.42) ng/mL,对照组分别为(206.89±45.01) ng/mL、(101.88±3.34 ) ng/mL,实验组血清及龈沟液中IL-35水平显著高于对照组( P<0.01)。实验组血清及龈沟液中IL-35水平分别与PD和CAL呈负相关。结论慢性牙周炎可导致血清及龈沟液中IL-35水平显著升高, 其升高程度与牙周炎呈负相关。

Expression and correlation of IL-35 in gingival crevicular fluid and serum with chronic periodontitis

PURPOSE: To investigate the concentration of IL-35 in serum and gingival crevicular fluid (GCF) and the correlation between IL-35 and periodontal clinical parameters, and explore the possible role of IL-35 in chronic periodontitis (CP). METHODS: Twenty patients with moderate or severe chronic periodontitis were selected as experimental group and 20 healthy people were included as control group. The patients’ baseline information, probing depth (PD) and clinical attachment level (CAL) were recorded. Their serum and GCF were collected and then the concentration of IL-35 in GCF and serum were detected by ELISA. SPSS19.0 software package was used for statistical analysis. RESULTS: The concentration of IL-35 in GCF and serum of chronic periodontitis were significantly higher than the control group (P<0.001). There was significant negative correlation between IL-35 and clinical parameters. CONCLUSIONS: Chronic periodontitis may increase the concentration of IL-35 in serum and GCF; IL-35 level is negatively correlated with periodontitis.