miR-1284过表达对人胃癌SGC-7901细胞基因表达谱及侵袭转移的影响

背景与目的：人胃癌组织微小RNA表达谱芯片筛选研究报道miR-1284与胃癌淋巴结转移有关，但其在胃癌中的具体作用鲜见报道。该研究探讨miR-1284过表达对人胃癌SGC-7901细胞基因表达谱及侵袭转移的影响。方法：以慢病毒介导miR-1284过表达转染胃癌SGC-7901细胞为实验组(LV-miR-1284组)，转染空载体慢病毒载体的细胞为阴性对照组(LV-NC-GFP组)，未转染慢病毒载体的细胞为空白组(Control组)。采用荧光定量PCR检测各组细胞miR-1284的表达，芯片检测各组细胞差异表达基因，生物信息学预测miR-1284的靶基因，Transwell侵袭实验检测各组细胞侵袭能力，裸鼠皮下移植瘤模型检测各组细胞转移能力。结果：PCR结果显示，与LV-NC-GFP组和Control组比较，LV-miR-1284组细胞miR-1284表达明显增高；芯片结果显示，LV-miR-1284组有20个基因表达显著上调，17个基因表达显著下调；生物信息学预测miR-1284的靶基因有138个基因；Transwell侵袭实验结果显示，LV-miR-1284组侵袭细胞数为70.00±2.37，其细胞侵袭能力较LV-NCGFP组(168.67±4.55)和Control组(170.33±3.08)明显减弱；体内裸鼠实验结果显示，LV-miR-1284组肝脏转移率为14.29%，其细胞转移能力较LV-NC-GFP组(85.71%)和Control组(85.71%)明显减弱。结论：miR-1284过表达能抑制人胃癌SGC-7901细胞侵袭转移，其机制可能与调控SUMO1、JUN基因表达有关。

The effect of miR-1284 over-expression on gene expression profiling and invasion/metastasis of human gastric cancer SGC-7901 cells

Background and purpose: It has been reported that miR-1284 is associated with gastric cancer lymph node metastasis in the research of microRNA microarray in human gastric cancer tissues. But the specific role of miR-1284 in gastric cancer has not been reported. The aim of this study was to investigate the effect of miR-1284 over-expression on the gene expression profiling and invasion/metastasis of human gastric cancer SGC-7901 cells. Methods: Gastric cancer SGC-7901 cells of LV-miR-1284 group were transfected with lentiviral vectors of miR-1284, cells of LV-NC-GFP group were transfected with lentiviral vectors without miR-1284, and cells of control group were not transfected with lentiviral vectors. The expression of miR-1284 was detected by the real-time fluorescent quantitative PCR. Differential expression genes were detected by the microRNA chip. Target genes of miR-1284 were predicted by the bioinformatics. Invasive ability was detected by the Transwell invasion assay. Metastasis ability was detected by subcutaneously transplanted tumor model of nude mice. Results: Compared with LV-NC-GFP and control groups, the expressions of miR-1284 and 20 genes were up-regulated, and the expression of 17 genes was down-regulated in LV-miR-1284 group. One hundred and thirty-eight target genes of miR-1284 were predicted by the bioinformatics website. Compared with invasive cell number of LV-NC-GFP group (168.67±4.55) and control group 170.33±3.08), the ability of invasion of cells was weakened in LV-miR-1284 group (70.00±2.37). Compared with the liver metastasis rate of LV-NC-GFP group (85.71%) and control group (85.71%), the ability of metastasis of cells was weakened in LV-miR-1284 group (14.29%). Conclusion: The ability of invasion and metastasis of SGC-7901 cells is suppressed by over-expression of miR-1284. The mechanism may be related to regulating the expression of SUMO1 and JUN genes.