Tau蛋白和磷酸化Tau蛋白在C57BL/6小鼠和转基因鼠的表达

目的　探讨总Ｔａｕ蛋白（Ｔ-ｔａｕ）和磷酸化Ｔａｕ蛋白（Ｐ-ｔａｕ）在慢性青光眼模型小鼠视神经中的表达以及意义。方法　选取Ｃ５７ＢＬ／６雄性小鼠以及ＡＰＰ＋转基因鼠各４０只，每只小鼠右眼为实验眼，左眼为对照眼，通过烙闭实验眼巩膜上静脉建立慢性青光眼模型，采用免疫组织化学方法，检测视神经Ｔ-ｔａｕ以及Ｐ-ｔａｕ在不同青光眼模型中的表达情况。结果　Ｃ５７ＢＬ／６小鼠以及ＡＰＰ＋转基因鼠实验眼术后眼压均高于术前（均为Ｐ＜０．０５），而两组术后眼压差异无统计学意义（Ｐ＞０．０５）。Ｃ５７ＢＬ／６小鼠以及ＡＰＰ＋转基因鼠实验眼Ｔ-ｔａｕＩＯＤ值均低于对照眼（均为Ｐ＜０．０５），而Ｐ-ｔａｕＩＯＤ值均高于对照眼（均为Ｐ＜０．０５）；Ｃ５７ＢＬ／６小鼠实验眼Ｔ-ｔａｕ和Ｐ-ｔａｕＩＯＤ值分别为１０２．２４±１２．１２和１７２．１２±９．０１，均小于ＡＰＰ＋转基因鼠（均为Ｐ＜０．０５）。ＡＰＰ＋转基因鼠实验眼视神经细胞凋亡率为（９．７２±１．０８）％，明显高于Ｃ５７ＢＬ／６小鼠实验眼（７．２４±１．２２）％，差异有统计学意义（均为Ｐ＜０．０５），且两者实验眼均高于对照眼（均为Ｐ＜０．０５）。结论　慢性青光眼小鼠视网膜Ｔ-ｔａｕ蛋白表达量降低，Ｔａｕ蛋白磷酸化水平增加，视神经细胞凋亡率上升，在ＡＰＰ＋转基因小鼠中尤为明显。

Expression of Tau protein and phosphorylation Tau protein in C57BL/6 and transgenic mice

Objective To investigate the expression of total Tau protein ( T-tau) and phosphorylation Tau protein ( P-tau) in optic nerve of chronic glaucoma mice model and its significance. Methods Forty C57BL/6 mice and APP + transgenic nuce were chosen .the right eye of each mouse as the experimental eye ,the left eye as control . the chronic glaucoma model was established by ligating four episcleral vein in the experimental eye, and the immunohistochemical staining method was used to detect the expression of T-tau and P-tau in the optic nerve. Results The postoperative intraocular pressure in the experimental eyes of C57BL/6 mice and APP+ transgenic mice were higher than those before operation ( all P < 0. 05 ) .while the difference in the postoperative intraocular pressure between C57BL/6 mice and APP+ transgemc mice was not statistical significant (P > 0. 05 ). IOD value of T-tau protein in the experimental eyes of C57BL/6 mice and APP+ transgenic mice were lower than that in the control eyes ( all P < 0. 05 ) ,while the IOD value of P-tau protein were higher than the control eyes ( all P < 0. 05 ) ;IOD values of T-tau and P-tau protein in the experimental eyes of C57BL/6 mouse were 102. 24 + 12. 12 and 172. 12 + 9. 01 , respectively , were less than APP + transgenic mice ( all P < 0. 05 ) . The apoptotic rate of optic nerve cell in the experimental eyes of APP+ transgenic mice was (9. 72 + 1. 08 ) % , significantly higher than that of C57BL/6 mice ( 7. 24 + 1. 22 ) % . the difference was statistically significant ( P < 0. 05 ) . and the experimental eyes were all higher than the control eye ( all P < 0. 05 ) . Conclusion The expression of T-tau protein in retina of chronic glaucoma mice decreases, Tau protein phosphorylation level increases , apoptotic rate of optic nerve cell increases , especially in APP+ transgenic mice.